Catalog #BE0131FAB
InVivoMAb anti-mouse CTLA-4 (CD152) F(ab) fragment
Clone
9H10 f(ab) Fragment
Reactivities
Mouse
Isotype
Syrian hamster IgG
Product Description
This product is an F(ab) fragment derived from the anti-mouse CTLA-4 antibody clone 9H10. The 9H10 monoclonal antibody reacts with mouse CTLA-4, also known as cytotoxic T lymphocyte antigen-4 or CD152. CTLA-4 is a 33 kDa cell-surface receptor encoded by the Ctla4 gene and belongs to the CD28 family of the immunoglobulin superfamily. CTLA-4 is expressed on activated T lymphocytes, regulatory T cells, and activated B lymphocytes. CTLA-4 is structurally similar to the T cell co-stimulatory receptor CD28, and both molecules bind the B7 family members B7-1 (CD80) and B7-2 (CD86). Upon ligand binding, CTLA-4 negatively regulates cell-mediated immune responses and plays important roles in immune tolerance, thymocyte development, and the regulation of protective immunity. Unlike a full-length antibody, this F(ab) fragment contains only the antigen-binding region and lacks the Fc domain. As a result, the fragment retains specific binding to mouse CTLA-4 while eliminating Fc-mediated effector functions such as complement activation and Fcγ receptor engagement. Because the Fc region is absent, this format is useful in experiments where CTLA-4 binding or receptor engagement is desired without Fc-dependent immune effects, including antibody-mediated cytotoxicity, Fcγ receptor–mediated cross-linking by accessory cells, or complement activation. CTLA-4 is an important inhibitory immune checkpoint receptor and is widely studied in the context of immune regulation, T cell activation, regulatory T cell biology, and cancer immunotherapy. The full-length 9H10 antibody has been shown to promote T cell co-stimulation by blocking CTLA-4 binding to the B7 co-receptors, thereby allowing CD28-mediated signaling. The F(ab) fragment format may provide a useful tool for studying CTLA-4 binding, receptor engagement, and checkpoint biology while minimizing secondary Fc-mediated mechanisms that can complicate interpretation of results. This anti-mouse CTLA-4 F(ab) fragment is well suited for studies focused on CTLA-4 targeting, immune checkpoint regulation, and CTLA-4 engagement without Fc-mediated effects.
Specifications
| Isotype | Syrian hamster IgG |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Mouse CTLA-4-human IgG1 fusion protein |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤1EU/mg (≤0.001EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE and SEC |
| Sterility | 0.2 µm filtration |
| Production | Papain Digest |
| Purification | Protein G |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |