Catalog #SIM0046
InVivoSIM anti-human CTLA-4 (Tremelimumab Biosimilar)
Clone
Tremelimumab
Reactivities
Human
Applications
CTLA-4 neutralization
Flow Cytometry
ELISA
Western Blot
Flow Cytometry
ELISA
Western Blot
Isotype
Human IgG2, κ
Product Description
This biosimilar antibody uses the same variable regions from the therapeutic antibody Tremelimumab making it ideal for research use. This Tremelimumab biosimilar is a fully human monoclonal antibody which reacts with human CTLA-4 (cytotoxic T lymphocyte antigen-4) also known as CD152. CTLA-4 is a 33 kDa cell surface receptor encoded by the Ctla4 gene that belongs to the CD28 family of the Ig superfamily. CTLA-4 is expressed on activated T and B lymphocytes. CTLA-4 is structurally similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to the B7 family members B7-1 (CD80) and B7-2 (CD86). Upon ligand binding, CTLA-4 negatively regulates cell-mediated immune responses. CTLA-4 plays roles in induction and/or maintenance of immunological tolerance, thymocyte development, and regulation of protective immunity. CTLA-4 is among a group of inhibitory receptors being used as cancer treatment targets through immune checkpoint blockade. Tremelimumab binds to CTLA-4, blocking the inhibitory signal, which allows the cytotoxic T cells to kill cancer cells. Tremelimumab and Ipilimumab are both fully human monoclonal antibodies directed against CTLA-4, but Ipilimumab is an IgG1 isotype and Tremelimumab is a non-complement-fixing IgG2 isotype.
Specifications
| Isotype | Human IgG2, κ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | S378A |
| Immunogen | Human CTLA-4 |
| Reported Applications |
CTLA-4 neutralization Flow Cytometry ELISA Western Blot |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| RRID | AB_3696385 |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | This product should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |