Antibody Production Services

At Bio X Cell, we know that growing hybridoma cells and purifying antibodies in large quantities with low endotoxin levels can be technically challenging and time-consuming.

Enlist our experienced team to do this for you! Bio X Cell’s optimized fermentation process produces more antibody in less time than traditional methods. A technical project manager will guide you through the process, providing recommendations and updates along the way.

Project Workflow

1. Pilot Instructions for preparing and shipping cells.
Assessment of cell line productivity: >30mg of antibody/Liter is required to proceed to scale-up, and a pilot fee will be charged.
Mycoplasma testing service.
Isotyping of cell culture supernatant.
2. Fermentation Cell culturing and monitoring 7 days/week.
Freezing of five vials of cells.
Storage of cells at Bio X Cell for rapid re-supply.
Expansion of cells using optimized fermentation process.
Concentration of cell supernatant.
3. Purification Purification of supernatant on Protein A or G.
Buffer exchange to PBS that is preservative and
Sterile filtering of final product.
  • Testing to confirm <2EU/mg endotoxin.
  • <1EU/mg available for an additional charge.
4. Vialing and QC
  • Sterile vialing of finished product.
  • Custom aliquoting available for an additional charge.
Internal antibody quality assessment.
Certificate of Analysis (CoA).
Murine pathogen screening service.
5. Shipping Shipping on cold packs in environmentally friendly packaging.

"I am totally satisfied with the service Bio X Cell has given us. When anyone asks me for CTLA-4 antibodies, I always refer them to you. I look forward to another 20 years."

Customer Testimonials