Your Hybridoma or Cell Line.
Purified Antibody Back to You in 4 to 6 Weeks.
Endotoxin-controlled contract antibody manufacturing for established preclinical programs. Milligram to multi-gram lots. U.S.-based production. Identical formulation and QC standards from pilot through program scale. Hybridoma scale-up, antibody production from stable cell lines, and recombinant source manufacturing available.
Antibody Production and Manufacturing Designed for Mid-Scale Programs
Bio X Cell was built for the scale between discovery and clinical: 25 mg to multi-gram lots, endotoxin-controlled, in vivo-ready, with an average 4 to 6 week turnaround. The platform behind ~30,000 peer-reviewed citations, available for your custom program.
Production workflow and antibody delivery
Transfer starting project material
Ship hybridoma cells, stable cell line, or defined production materials. Bio X Cell immediately initiates production. Communication for a dedicated project manager throughout. Optional cell banking keeps the line secure for seamless resupply.
Define requirements and confirm specifications
Provide starting material details, target lot size, formulation requirements, and study timeline. Bio X Cell confirms the production plan and endotoxin specifications at project scoping. No ambiguity at lot release.
Receive purified
in vivo-ready reagents
Protein A or G purification, buffer exchange to PBS (no preservatives, no stabilizers), sterile filtration. Antibody formulated to client specifications, custom-vialed, and shipped with a per-lot Certificate of Analysis. Average 4 to 6 weeks from receipt of starting material.
Hybridoma Scale-Up and Cell Line Services
Bio X Cell manages your hybridoma or stable cell line from transfer through purified antibody delivery, including support preparing and shipping your cells to the facility.
Cell banking and storage
Scale-up and purification
Isotype confirmation
Mycoplasma and pathogen testing
Antibody Production Capabilites for Preclinical Research
| Phase | Description | Included | Optional Add-Ons |
|---|---|---|---|
| Pilot | Instructions for preparing and shipping cells |  |  |
| Small-scale assessment of cell line activity with Protein A or G purification | | |
|
| Mycoplasma testing | | |
|
| Antibody isotyping | | |
|
| Cell Culture | Cell culturing and monitoring (7 days/week) | | |
| Expansion of cells using optimized culture processes | | |
|
| Freezing of five vials of cells (supports future production continuity) | | |
|
| Storage of cells at Bio X Cell (enables rapid re-supply) | | |
|
| Purification | Purification of supernatant using Protein A or G | | |
| Buffer exchange to PBS (preservative- and stabilizer-free) | | |
|
| Sterile filtration of final product | | |
|
| Endotoxin assessment (&le1 EU/mg standard; further purification available) | | |
|
| Vialing & QC | Sterile vialing of finished product | | |
| Custom aliquoting | | |
|
| Internal antibody quality assessment | | |
|
| Certificate of Analysis (CoA) | | |
|
| Murine pathogen screening | | |
|
| Aggregation analysis by SEC (aggregate profile characterization) | | |
|
| Shipping | Cold-pack shipping in environmentally friendly packaging | | |
Contract Antibody Production Services: Frequently Asked Questions
Purified, characterized antibody lots. A per-lot Certificate of Analysis documenting all QC results ships with the antibody.
Yes. Provide your hybridoma cells and Bio X Cell handles cell banking, culture scale-up, Protein A or G purification, QC testing, and delivery of purified antibody lots. Pilot production is available before committing to full-scale runs. Cell banking secures the line for ongoing supply.
Bio X Cell routinely achieves endotoxin levels as low as ≤0.1 EU/mg. Endotoxin is controlled during manufacturing, not corrected after purification. Program-defined specifications for sensitive model systems are set at project scoping and tested on every lot before release.
Bio X Cell supports production from 25 mg up to multi-gram quantities. Lot size is defined by program requirements and confirmed during project scoping.
Large CDMOs are optimized for clinical-scale GMP manufacturing. Bio X Cell serves the mid-scale research use only layer: 25 mg to multi-gram lots, research-use cost profile, endotoxin-controlled manufacturing, and 4 to 6 week average turnaround. Over 1,000 custom projects completed.
Contact Bio X Cell to confirm starting material, target lot size, and study specifications. A Bio X Cell scientist works with you from the start, guiding cell preparation and shipment through to target specifications and QC. Projects begin within days of starting material receipt, with an average 4 to 6 week turnaround.
No. Bio X Cell does not provide antibody discovery, immunization, or hybridoma generation. Bio X Cell engineers and produces antibodies from an existing starting point: your sequence, construct, hybridoma, or a Bio X Cell catalog clone.
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Custom Antibody Services
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Whether you need antibody customization or high-volume production, Bio X Cell is committed to advancing your therapeutic innovations.
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