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Antibody Production from Hybridoma & Stable Cell Lines
Antibody production with expert support & superior quality
The experts at Bio X Cell manage the complexity of expanding hybridoma cells and purifying antibodies in large quantities while maintaining low endotoxin levels. By leveraging our optimized cell culture processes, we produce antibodies more efficiently than traditional methods, helping you meet your demanding project timelines.
You’ll have a dedicated technical project manager by your side throughout the entire journey, ensuring clear communication and guidance at every stage. Antibody production is conducted in the U.S. at our Lebanon, New Hampshire facility, enabling seamless communication, swift turnaround times, and superior quality.
25+ years of experience
Ultra-low endotoxin
Animal-free facility
Superior lot-to-lot consistency
Scalable production
Rapid turnaround
Customizable services
Antibody Production & Purification Services
Our US-based facility, with 26,000 ft2 of manufacturing space, enables high-volume antibody manufacturing – up to 2,000L per month. Therefore, based on your research program needs, we can scale production from milligrams to grams. We fully control our supply chain from end to end, assuring lot-to-lot consistency. In short, your program will never be delayed due to capacity or throughput constraints.
Suspension cultures onlyAdditional Antibody Services
Cell freezing and storage, mycoplasma testing, and isotyping capabilities allow us to customize your program’s services to best meet your needs.
Antibody Production Project Workflow
| Phase | Description | Included | Optional Add-Ons |
|---|---|---|---|
| Pilot | Instructions for preparing and shipping cells |  |  |
| Assessment of cell line activity utilizing a small-scale cell culture followed by either protein A or G affinity purification | | |
|
| Mycoplasma testing service | | |
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| Antibody isotyping service | | |
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| Cell Culture | Cell culturing and monitoring 7 days/week | | |
| Freezing of five vials of cells | | |
|
| Storage of cells at Bio X Cell for rapid re-supply | | |
|
| Expansion of cells using optimized cell culture process | | |
|
| Purification | Purification of supernatant on Protein A or G | | |
| Buffer exchange to PBS which is preservative and stabilizer-free | | |
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| Sterile filtering of final product | | |
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| Testing to confirm ≤1 EU/mg endotoxin levels; additional testing and antibody purification services available | | |
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| Vialing & QC | Sterile vialing of finished product | | |
| Custom aliquoting available | | |
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| Internal antibody quality assessment | | |
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| Certificate of Analysis | | |
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| Murine pathogen screening service | | |
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| Aggregation analysis by SEC | | |
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| Shipping | Shipping on cold packs in environmentally friendly packaging | | |
Explore Our Comprehensive Custom Antibody Solutions
Antibody Engineering & Recombinant Production Services
Antibody Conjugation Services
Antibody Quality Control & Testing Services
Consult With Bio X Cell to Enable Your Next Breakthrough Discovery
Whether you need antibody customization or high-volume production, Bio X Cell is committed to advancing your therapeutic innovations.
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