Home Dilution Calculator

Dilution Calculator

Calculate the Dilution Required To Prepare a Working Solution of Your Antibody

The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of antibody to a known concentration. Enter C1, C2 and V2 to calculate V1.


The Dilution Calculator Equation

The dilution calculator is based on the following equation:

Concentration(start) x Volume(start) = Concentration(final) x Volume(final)

This is the C1V1 = C2V2 equation.

An Example of a Dilution Calculation Using the Dilution Calculator

You received an antibody at a stock concentration of 10 mg/ml. You have five mice to inject with 200 µg per mouse. You want to inject each mouse with a 200 µL injection volume. This means you will need 1,000 µg in 1,000 µL (1 milliliter of a 1 mg/ml solution).

Using the equation C1V1 = C2V2, where C1= 10 mg/ml, C2= 1 mg/ml, V2= 1 ml and V1 is the unknown:

  1. Enter 10 in the Concentration (start) box and select the correct unit (mg/ml).
  2. Enter 1 in the Concentration (final) box and select the correct unit (mg/ml).
  3. Enter 1,000 in the Volume (final) box and select the correct unit (microliter).
  4. Press calculate.
  5. The answer of 100 microliter (0.1 ml) appears in the Volume (start) box.