Catalog #BE0001-2FAB
InVivoMAb anti-human CD3 F(ab) fragment
Clone
OKT-3 f(ab) Fragments
Reactivities
Human
Isotype
Mouse IgG2a, κ
Product Description
This product is an F(ab) fragment derived from the anti-human CD3 antibody clone OKT-3. CD3 is a multi-subunit signaling complex associated with the T cell receptor (TCR) and is expressed on nearly all mature T lymphocytes. Engagement of CD3 plays a central role in T cell activation by transmitting signals from the TCR following antigen recognition. Unlike a full-length antibody, this F(ab) fragment contains only the antigen-binding region and lacks the Fc domain. As a result, the fragment retains specific binding to CD3 while eliminating Fc-mediated effector functions such as complement activation and Fcγ receptor engagement. Because the Fc region is absent, this format is useful in experiments where CD3 engagement is desired without Fc-dependent immune effects, including antibody-mediated cytotoxicity, Fcγ receptor–driven cross-linking by accessory cells, or complement activation. F(ab) fragments can therefore provide a more controlled way to study TCR/CD3 signaling or receptor engagement while minimizing secondary immune mechanisms that can complicate interpretation of results. F(ab) fragments targeting CD3 may also be useful in emerging targeted delivery and cell-engineering strategies. For example, CD3-binding fragments can be used to coat nanoparticles, viral vectors, or other delivery systems to selectively target T cells and deliver genetic payloads such as CAR constructs or gene-editing components directly in vivo. By avoiding Fc-mediated immune interactions, F(ab) fragments help preserve target cells while enabling receptor-specific targeting and delivery. This anti-human CD3 F(ab) fragment therefore provides a useful reagent for studies focused on T cell targeting, CD3 engagement without Fc-mediated effects, and receptor-directed delivery approaches.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Human CD3ε |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤1EU/mg (≤0.001EU/μg) Determined by LAL assay |
| Aggregation* |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE and SEC |
| Sterility | 0.2 µm filtration |
| Production | Pepsin Digest |
| Purification | Protein G |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |