Catalog #CPB538
Bispecific anti-mouse CD3 x anti-mouse/human BCMA (D265A)
Clone
derived from clones 145-2C11 and Ab-2
Reactivities
Mouse, Human
Isotype
mouse IgG1 (D256A), κ
Product Description
CPB538 is a recombinant 1+1 bivalent T-cell–redirecting bispecific antibody structured after Erlanatamab but designed to simultaneously bind murine CD3ε and mouse/human BCMA (TNFRSF17/CD269). By bringing CD3-positive murine T cells into close proximity with BCMA-expressing target cells, this molecule is intended to promote target-dependent T-cell activation, immune-synapse formation, and cytotoxic elimination of BCMA-positive plasma-cell or myeloma-model targets. The D265A Fc substitution is incorporated to attenuate Fc-mediated interactions—particularly complement activation and Fcγ receptor–dependent effector activity—so that functional activity is driven predominantly by the CD3 × BCMA bridging mechanism rather than nonspecific Fc engagement. Use of a mouse IgG1 isotype prevents anti-drug immune responses in immunocompetent mouse models, enabling more interpretable repeat-dose pharmacology, efficacy, and tolerability studies. This reagent is useful for preclinical evaluation of BCMA-directed T-cell redirection, including target-dependent T-cell activation and cytotoxicity, BCMA-positive plasma-cell or myeloma-cell depletion, and efficacy and tolerability studies in immunocompetent mouse models expressing mouse or human BCMA.
Specifications
| Isotype | Mouse IgG1 (D265A) |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG1 (D265A) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | D265A |
| Reported Applications |
ELISA For information on in-vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 145.7 |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |