Catalog #CP043
RecombiMAb anti-mouse CD28 (LALA-PG)
Clone
D665-CP043
Reactivities
Mouse
Isotype
Mouse IgG2a
(switched from mouse IgG1)
(switched from mouse IgG1)
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Product Description
The D665-CP043 monoclonal antibody is a chimeric version of the original D665 antibody. The variable domain sequences are identical to the original D665 but the constant region sequences have been switched from mouse IgG1 to mouse IgG2a. The D665-CP043 antibody also contains a LALA-PG mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. The D665-CP043 monoclonal antibody reacts with mouse CD28, a 45 kDa costimulatory receptor and a member of the Ig superfamily. CD28 is expressed by thymocytes, most peripheral T cells, and NK cells. CD28 is a receptor for CD80 (B7-1) and CD86 (B7-2). Signaling through CD28 induces IL-2 and IL-2 receptor expression and T cell proliferation. The D665 antibody is a CD28 superagonist and is most commonly used to induce the expansion of Treg cells in vivo in various mouse models of disease.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Mutations | LALA-PG |
| Immunogen | A20 cells expressing mouse CD28 and a recombinant mouse CD28-Ig fusion protein |
| Reported Applications |
in vivo T cell stimulation/activation* in vitro T cell stimulation/activation* *Reported for the original mouse IgG1 D665 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |