RecombiMAb anti-mouse CD28 (LALA-PG)

Catalog #CP043
Clone:
D665-CP043
Reactivities:
Mouse
Isotype:
Mouse IgG2a
(switched from mouse IgG1)

$500.00

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Product Details

The D665-CP043 monoclonal antibody is a chimeric version of the original D665 antibody. The variable domain sequences are identical to the original D665 but the constant region sequences have been switched from mouse IgG1 to mouse IgG2a. The D665-CP043 antibody also contains a LALA-PG mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. The D665-CP043 monoclonal antibody reacts with mouse CD28, a 45 kDa costimulatory receptor and a member of the Ig superfamily. CD28 is expressed by thymocytes, most peripheral T cells, and NK cells. CD28 is a receptor for CD80 (B7-1) and CD86 (B7-2). Signaling through CD28 induces IL-2 and IL-2 receptor expression and T cell proliferation. The D665 antibody is a CD28 superagonist and is most commonly used to induce the expansion of Treg cells in vivo in various mouse models of disease.

Specifications

Isotype Mouse IgG2a, κ
Recommended Isotype Control(s) RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Mutations LALA-PG
Immunogen A20 cells expressing mouse CD28 and a recombinant mouse CD28-Ig fusion protein
Reported Applications in vivo T cell stimulation/activation*
in vitro T cell stimulation/activation*
*Reported for the original mouse IgG1 D665 antibody
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
Aggregation <5%
Determined by SEC
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from HEK293 cell supernatant in an animal free facility
Purification Protein G
Molecular Weight 150 kDa
Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

Additional Formats

See the references for the original mouse IgG1 D665 antibody (https://bioxcell.com/catalogsearch/result/?q=BE0328).