RecombiMAb anti-mouse Ly6G/Ly6C (Gr-1)
Catalog #CP172
Clone:
RB6-8C5-CP172
Reactivities:
Mouse
Isotype:
Mouse IgG2b
(switched from rat IgG2b)
(switched from rat IgG2b)
Product Details
The RB6-8C5-CP172 monoclonal antibody is a chimeric version of the original RB6-8C5 antibody. The variable domain sequences are identical to the original RB6-8C5 but the constant region sequences have been switched from rat IgG2b to mouse IgG2b. The RB6-8C5-CP172 antibody contains no Fc mutations just as the original rat IgG2b antibody does not. The RB6-8C5-CP172 monoclonal antibody reacts strongly with mouse Ly6G and weakly with mouse Ly6C previously referred to as GR-1. Ly6G is a 21-25 kDa member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Ly6G is expressed differentially during development by cells in the myeloid lineage including monocytes macrophages granulocytes and neutrophils. Monocytes typically express Ly6G transiently during development while mature granulocytes and peripheral neutrophils retain expression making Ly6G a good cell surface marker for these populations.The original RB6-8C5 antibody has been shown to deplete Gr-1 expressing cells.Specifications
| Isotype | Mouse IgG2b, Īŗ |
|---|---|
| Recommended Isotype Control(s) | InVivoPlus mouse IgG2b isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Mouse granulocytes |
| Reported Applications |
in vivo depletion of Gr-1+ myeloid cells* Flow cytometry* Immunohistochemistry (paraffin)* Immunohistochemistry (frozen)* *Reported for the original rat IgG2b RB6-8C5 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<1EU/mg (<0.001EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from CHO cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Additional Formats
Recommended Products
-
Recommended Isotype Control(s)
InVivoPlus mouse IgG2b isotype control, unknown specificity
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
See the references for the original rat IgG2b RB6-8C5 antibody (https://bioxcell.com/catalogsearch/result/?q=BP0075).
