RecombiMAb anti-mouse CD8α

Catalog #CP134
Clone:
YTS 169.4-CP134
Reactivities:
Mouse

$224.00 - $6,087.00

$224.00 - $6,087.00

Choose an Option...
  • 100 mg - $6,087.00
  • 50 mg - $4,356.00
  • 25 mg - $3,030.00
  • 5 mg - $868.00
  • 1 mg - $224.00
  • Custom Amount (Quotes Only)
In stock
Only %1 left
Isotype:
Mouse IgG2a
(switched from rat IgG2b)

Product Details

The YTS 169.4-CP134 monoclonal antibody is a chimeric version of the original YTS 169.4 antibody. The variable domain sequences are identical to the original YTS 169.4 but the constant region sequences have been switched from rat IgG2b to mouse IgG2a. The YTS 169.4-CP134 antibody contains no Fc mutations just as the original rat IgG2b antibody does not. The YTS 169.4-CP134 monoclonal antibody reacts with mouse CD8α. The CD8 antigen is a transmembrane glycoprotein that acts as a co-receptor for the T cell receptor (TCR). Like the TCR, CD8 binds to class I MHC molecules displayed by antigen presenting cells (APC). CD8 is primarily expressed on the surface of cytotoxic T cells, but can also be found on thymocytes, natural killer cells, and some dendritic cell subsets. CD8 most commonly exists as a heterodimer composed of one CD8α and one CD8β chain however, it can also exist as a homodimer composed of two CD8α chains. Both the CD8α and CD8β chains share significant homology to immunoglobulin variable light chains. The molecular weight of each CD8 chain is approximately 34 kDa. The YTS 169.4 antibody exhibits depleting activity when used in vivo.

Specifications

Isotype Mouse IgG2a, κ
Recommended Isotype Control(s) InVivoPlus mouse IgG2a isotype control, unknown specificity
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Conjugation This product is unconjugated. Conjugation is available via our Antibody Conjugation Services.
Immunogen CBA mouse thymocytes
Reported Applications in vivo CD8+ T cell depletion*
Western blot*
*Reported for the original rat IgG2b YTS 169.4 antibody
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
Aggregation <5%
Determined by SEC
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from CHO cell supernatant in an animal-free facility
Purification Protein G
Molecular Weight 150 kDa
Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

Additional Formats

See the references for the original rat IgG2b YTS 169.4 antibody (https://bioxcell.com/catalogsearch/result/?q=BP0117).