RecombiMAb anti-mouse/human CD11b
Catalog #CP051
Clone:
M1/70-CP051
Reactivities:
Mouse, Human
Isotype:
Mouse IgG2a
(switched from rat IgG2b)
(switched from rat IgG2b)
Product Details
The M1/70-CP051 monoclonal antibody is a chimeric version of the original M1/70 antibody. The variable domain sequences are identical to the original M1/70 but the constant region sequences have been switched from rat IgG2b to mouse IgG2a. The M1/70-CP051 monoclonal antibody reacts with mouse and human CD11b, a 170 kDa membrane glycoprotein also known as integrin alpha M (ITGAM). CD11b belongs to the integrin alpha family and is primarily expressed on granulocytes and monocytes/macrophages but also expressed on dendritic cells, NK cells, and subsets of T and B cells. CD11b and CD18 combine to form Mac-1. Mac-1 functions as a complement receptor as well as a receptor for fibrinogen, factor X, and ICAM1.Specifications
Isotype | Mouse IgG2a, Īŗ |
---|---|
Recommended Isotype Control(s) | InVivoPlus mouse IgG2a isotype control, unknown specificity |
Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
Immunogen | B10 mouse spleen cells enriched for T cells |
Reported Applications |
in vivo CD11b neutralization* ILC2 cell purification* Flow cytometry* *Reported for the original rat IgG2b M1/70 antibody |
Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
Endotoxin |
<1EU/mg (<0.001EU/Ī¼g) Determined by LAL gel clotting assay |
Aggregation |
<5% Determined by SEC |
Purity |
>95% Determined by SDS-PAGE |
Sterility | 0.2 Āµm filtration |
Production | Purified from HEK293 cell supernatant in an animal-free facility |
Purification | Protein G |
Molecular Weight | 150 kDa |
Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Additional Formats
Recommended Products
See the references for the original rat IgG2b M1/70 antibody (https://bioxcell.com/be0007).