Catalog #CP051
RecombiMAb anti-mouse/human CD11b
Clone
M1/70-CP051
Reactivities
Mouse, Human
Isotype
Mouse IgG2a, κ
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Product Description
The M1/70-CP051 monoclonal antibody is a chimeric version of the original M1/70 antibody. The variable domain sequences are identical to the original M1/70 but the constant region sequences have been switched from rat IgG2b to mouse IgG2a. The M1/70-CP051 monoclonal antibody reacts with mouse and human CD11b, a 170 kDa membrane glycoprotein also known as integrin alpha M (ITGAM). CD11b belongs to the integrin alpha family and is primarily expressed on granulocytes and monocytes/macrophages but also expressed on dendritic cells, NK cells, and subsets of T and B cells. CD11b and CD18 combine to form Mac-1. Mac-1 functions as a complement receptor as well as a receptor for fibrinogen, factor X, and ICAM1.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | InVivoPlus mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | B10 mouse spleen cells enriched for T cells |
| Reported Applications |
in vivo CD11b neutralization* ILC2 cell purification* Flow cytometry* *Reported for the original rat IgG2b M1/70 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |