RecombiMAb anti-mouse CD71 (TfR1)
Catalog #CP078
Clone:
8D3-CP078
Reactivities:
Mouse
Isotype:
Mouse IgG2a LALA-PG, kappa
(switched from rat IgG2a, kappa)
(switched from rat IgG2a, kappa)
Product Details
The 8D3-CP078 monoclonal antibody is a recombinant, chimeric version of the original 8D3 antibody. The variable domain sequences are identical to the original 8D3 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a, kappa for use in murine models. Additionally, 8D3-CP078 contains LALA-PG mutations in the heavy chain Fc fragment rendering it unable to bind endogenous murine FcĪ³ receptors or C1q to induce antibody-dependent, cell-mediated cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC). Species-matched chimeric antibodies result in reduced immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies. In vivo studies using a murine chimeric 8D3 antibody showed rapid uptake in the brain at a level comparable to the rat 8D3 Mab. Anti-TfR1 antibodies with eliminated Fc effector function have reduced acute clinical signs and reticulocyte depletion. Furthermore, murine studies using a TfR-targeting bispecific antibody showed that chimeric mouse IgG2a LALA-PG effector silencing demonstrated improved safety profiles. The LALA-PG variant had less effector function, C1q binding and C3 fixation compared to other common Fc silencing mutations such as the LALA and DANG variants.The 8D3-CP078 monoclonal antibody reacts with native, soluble and denatured forms of murine CD71, also known as transferrin receptor protein 1 (TfR1). CD71 is a 170-180 kDa type II homodimeric transmembrane glycoprotein expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation and is required for iron import from transferrin into cells by endocytosis. Cells of the vascular endothelium of brain capillaries that compose the blood-brain barrier (BBB) express high levels of TfR1 allowing for receptor-mediated transcytosis of large biomolecules into the brain. Murine chimeric anti-TfR1 8D3 has been used as a BBB transporter in mice and is suitable for studying CD71 expression and iron uptake into different tissues.
Specifications
| Isotype | Mouse IgG2a LALA-PG |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | LALA-PG |
| Immunogen | Mouse transformed endothelioma cell line t.end1 |
| Reported Applications |
Targeted drug delivery to the brain Immunohistochemistry Flow Cytometry Western Blot Transport across the BBB Receptor mediated transcytosis |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<1EU/mg (<0.001EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Additional Formats
Recommended Products
-
Recommended Isotype Control(s)
RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer