FlowMAb APC anti-human CD3

Catalog #FM0001-2-APC
Clone:
OKT-3
Reactivities:
Human
As low as $33.00

$33.00 - $77.00

$33.00 - $77.00

Choose an Option...
  • 100 µg - $77.00
  • 25 µg - $33.00
In stock
Only %1 left

Product Details

The OKT-3 monoclonal antibody reacts with human CD3ε, a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3ε is one of five polypeptide chains that combine to form the TCR complex. CD3ε is expressed on T lymphocytes, NK-T cells, and, to varying degrees, on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The OKT3 antibody clone can block the binding of clones SK7 and UCHT1. This allophycocyanin (APC)-conjugated version of the OKT-3 antibody is useful for flow cytometry.

Specifications

Isotype Mouse IgG2a, κ
Recommended Isotype Control(s) FlowMAb APC mouse IgG2a isotype control, unknown specificity
Conjugation APC
Excitation Source Red 627-640 nm
Excitation Max 651 nm
Emission Max 660 nm
Immunogen Not available or unknown
Reported Applications Flow cytometry
Formulation PBS, pH 7.0
Contains 0.09% Sodium Azide
Production Purified from cell culture supernatant in an animal-free facility
Purification Protein G. Conjugated with allophycocyanin under optimal conditions.
Storage The antibody solution should be stored at the stock concentration at 4°C and protected from prolonged exposure to light. Do not freeze.
Flow Cytometry
Willing, A., et al. (2014). "CD8(+) MAIT cells infiltrate into the CNS and alterations in their blood frequencies correlate with IL-18 serum levels in multiple sclerosis" Eur J Immunol 44(10): 3119-3128. PubMed

Recent findings indicate a pathogenic involvement of IL-17-producing CD8(+) T cells in multiple sclerosis (MS). IL-17 production has been attributed to a subset of CD8(+) T cells that belong to the mucosal-associated invariant T (MAIT) cell population. Here, we report a reduction of CD8(+) MAIT cells in the blood of MS patients compared with healthy individuals, which significantly correlated with IL-18 serum levels in MS patients. In vitro stimulation of peripheral blood mononuclear cells from healthy individuals and MS patients with IL-18 specifically activated CD8(+) MAIT cells. Moreover, IL-18 together with T-cell receptor stimulation induced, specifically on CD8(+) MAIT cells, an upregulation of the integrin very late antigen-4 that is essential for the infiltration of CD8(+) T cells into the CNS. Notably, we were able to identify CD8(+) MAIT cells in MS brain lesions by immunohistochemistry while they were almost absent in the cerebrospinal fluid (CSF). In summary, our findings indicate that an IL-18-driven activation of CD8(+) MAIT cells contributes to their CNS infiltration in MS, in turn leading to reduced CD8(+) MAIT-cell frequencies in the blood. Therefore, CD8(+) MAIT cells seem to play a role in the innate arm of immunopathology in MS.