RecombiMAb anti-rat/mouse CD71 (TfR1)
Catalog #CP079
Clone:
OX-26-CP079
Reactivities:
Mouse, Rat
Isotype:
mouse IgG2a LALA-PG, kappa
Product Details
The OX-26-CP079 monoclonal antibody is a recombinant version of the original OX-26 antibody that contains the Fc-silencing mutations in the heavy chain rendering it unable to bind endogenous murine FcĪ³ receptors or C1q. The LALA-PG mutations prevent antibody-dependent, cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). The LALA-PG variant has significantly reduced effector function, C1q binding and C3 fixation compared to other common silencing mutations such as the LALA and DANG variants while retaining favorable biophysical and manufacturing properties. Recent studies have demonstrated that administration of anti-TfR1 antibodies with Fc effector function in mice resulted in acute clinical signs of toxicity and a decrease in reticulocyte count. These negative effects were eliminated through Fc-silencing.The OX-26 monoclonal antibody reacts with rat and mouse CD71, also known as transferrin receptor protein 1 (TfR1). CD71 is a type II homodimeric transmembrane glycoprotein which is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation and is required for iron import from transferrin into cells by endocytosis. CD71 is overexpressed on many different types of cancer cells and expression level correlates with advanced stage and/or poorer prognosis in several cancers, including solid cancers. Elevated levels of CD71 expression on malignant cells, together with its extracellular accessibility, ability to internalize, and central role in cancer cell pathology make this receptor an attractive target for antibody-mediated therapy. In addition, cells of the vascular endothelium of brain capillaries that compose the blood-brain barrier (BBB) also express high levels of CD71 allowing for receptor-mediated transcytosis of large biomolecules into the brain. OX-26 has been used as a BBB transporter in rats and mice and is suitable for studying CD71 expression and iron uptake into different tissues. The OX-26 antibody binds to a distinct extracellular epitope on TfR that does not compete for binding with the natural ligand Tf or iron uptake. The OX-26 antibody is often used to transport conjugated drugs across the BBB in experimental models.
Specifications
| Isotype | Mouse IgG2a LALA-PG |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | LALA-PG |
| Immunogen | PHA-activated PVG rat lymph node cells |
| Reported Applications |
Targeted drug delivery to the brain Immunohistochemistry Flow Cytometry *Reported for the original mouse IgG1 antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<1EU/mg (<0.001EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Additional Formats
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer