RecombiMAb anti-rat/mouse CD71 (TfR1)
Catalog #CP059
Clone:
OX-26-CP059
Isotype:
Rat IgG2a, kappa
(switched from mouse IgG2a, kappa)
(switched from mouse IgG2a, kappa)
Product Details
The OX-26-CP059 monoclonal antibody is a recombinant, chimeric version of the original OX-26 antibody. The variable domain sequences are identical but the constant region sequences have been switched from mouse IgG2a, kappa to rat IgG2a, kappa for use in rat models. Species-matched chimeric antibodies exhibit regulated effector functionsāincluding Fc receptor binding and complement activationāand result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.The OX-26 monoclonal antibody reacts with rat and mouse CD71, also known as transferrin receptor protein 1 (TfR1). CD71 is a type II homodimeric transmembrane glycoprotein which is expressed on the surface of proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation and is required for iron import from transferrin into cells by endocytosis. CD71 is overexpressed on many different types of cancer cells and expression level correlates with advanced stage and/or poorer prognosis in several cancers, including solid cancers. Elevated levels of CD71 expression on malignant cells, together with its extracellular accessibility, ability to internalize, and central role in cancer cell pathology make this receptor an attractive target for antibody-mediated therapy. The anti-tumor and cytotoxic activities of anti-TfR1 antibodies have been demonstrated to require Fc effector function to elicit antibody-dependent cell-mediated cytotoxicity and antibody-dependent cell-mediated phagocytosis. In addition, cells of the vascular endothelium of brain capillaries that compose the blood-brain barrier (BBB) also express high levels of CD71 allowing for receptor-mediated transcytosis of large biomolecules into the brain. OX-26 has been used as a BBB transporter in rats and mice and is suitable for studying CD71 expression and iron uptake into different tissues. The OX-26 antibody is often used to transport conjugated drugs across the BBB in experimental models.
Specifications
| Isotype | Rat IgG2a |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb rat IgG2a isotype control, anti-trinitrophenol |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | PHA-activated PVG rat lymph node cells |
| Reported Applications |
Targeted drug delivery to the brain Immunohistochemistry Flow Cytometry *Reported for the original mouse IgG1 antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<1EU/mg (<0.001EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Additional Formats
Recommended Products
-
Recommended Isotype Control(s)
InVivoMAb rat IgG2a isotype control, anti-trinitrophenol
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
Please see references for the original mouse IgG2a OX-26 antibody (https://bioxcell.com/invivomab-anti-rat-mouse-cd71-tfr1-be0331).