RecombiMAb anti-mouse Ly6G

Catalog #CP129
Clone:
1A8-CP129
Reactivities:
Mouse
Isotype:
Mouse IgG2a
(switched from rat IgG2a)

$217.00 - $5,910.00

Choose an Option...
  • 100 mg - $5,910.00
  • 50 mg - $4,229.00
  • 25 mg - $2,942.00
  • 5 mg - $843.00
  • 1 mg - $217.00
  • Custom Amount (Quotes Only)
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Product Details

The 1A8-CP129 monoclonal antibody is a chimeric version of the original 1A8 antibody. The variable domain sequences are identical to the original 1A8 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a. The 1A8-CP129 antibody contains no Fc mutations just as the original rat IgG2a antibody does not. The 1A8-CP129 monoclonal antibody reacts with mouse Ly6G. Ly6G is a 21-25 kDa member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Ly6G is expressed differentially during development by cells in the myeloid lineage including monocytes, macrophages, granulocytes, and neutrophils. Monocytes typically express Ly6G transiently during development while mature granulocytes and peripheral neutrophils retain expression making Ly6G a good cell surface marker for these populations. Unlike the RB6-8C5 antibody, the 1A8-CP129 antibody reacts specifically with mouse Ly6G with no reported cross reactivity with Ly6C.

Specifications

Isotype Mouse IgG2a, κ
Recommended Isotype Control(s) InVivoPlus mouse IgG2a isotype control, unknown specificity
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen EL4J cells transfected with Ly6G
Reported Applications in vivo neutrophil depletion*
in vivo MDSC depletion*
Immunofluorescence*
Immunohistochemistry (paraffin)*
Immunohistochemistry (frozen)*
Flow cytometry*
*Reported for the original rat IgG2a 1A8 antibody
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
Aggregation <5%
Determined by SEC
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from CHO cell supernatant in an animal free facility
Purification Protein G
Molecular Weight 150 kDa
Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

Additional Formats

See the references for the original rat IgG2a 1A8 antibody (https://bioxcell.com/catalogsearch/result/?q=BP0075-1).