InVivoSIM anti-human TROP-2 (Sacituzumab Biosimilar)
Catalog #SIM0051
Clone:
Sacituzumab
Reactivities:
Human
Product Details
This biosimilar antibody uses the same variable regions as the therapeutic antibody Sacituzumab, making it ideal for research use. Sacituzumab is a fully humanized IgG1Īŗ monoclonal antibody that reacts with human trophoblastic cell surface antigen 2 (TROP-2), an antigen linked to cancer. TROP-2 is a cell surface receptor that spans the cellular membrane with an extracellular, transmembrane, and intracellular domain, along with a cytoplasmic tail essential for signaling. The ligands of TROP-2 include claudin-1, claudin-7, cyclin D1, and potentially IGF-1. TROP-2 upregulates EpCAM-triggered cell signaling and requires RIP to regulate efficient growth and division of cancer cells. Several proteins, including RIP, TACE, Ī³-secretase, Presenilin 1 (PS-1) and PS-2, facilitate TROP-2ās cleavage within the transmembrane domain, and this cleavage produces TROP-2 extracellular domain (ECD) and intracellular domain (ICD) fragments. TROP-2 is considered a cellular marker of trophoblastic stem cells, and this glycoprotein is known to transduce calcium signals. Normal epithelial cells express TROP-2 at a low level, while many tumors (such as glioblastoma, pancreatic carcinoma, and all breast cancer subtypes, especially triple-negative breast cancer) express it at a high level. The critical role of TROP-2 in multiple signaling pathways often links to the proliferation, invasion, and metastasis of tumors. The tumor cell-specific expression of TROP-2 makes it an ideal target for cancer immunotherapy. Several recent studies have used Sacituzumab-based antibody-drug conjugates (ADC), namely Sacituzumab Govitecan (IMMU-132) that contains Sacituzumab conjugated to SN-38 (the active metabolite of irinotecan), utilizing a pH hydrolysable linker CL2A for facilitating SN-38 release to the cancer cells in in vitro and in vivo preclinical studies.Specifications
| Isotype | Human IgG1, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG1 (K214R/L234F/L235E/P331S) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Mutations | K214R |
| Immunogen | Human TROP-2 |
| Reported Applications |
in vitro functional assays in vivo functional assays Antibody-drug conjugate synthesis ELISA Western blot Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG1 (K214R/L234F/L235E/P331S) isotype control, anti-hen egg lysozyme
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
