InVivoMAb anti-human TROP-2

Catalog #BE0408
Clone:
Pr1E11
Reactivities:
Human

$159.00 - $4,155.00

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  • 100 mg - $4,155.00
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Product Details

The Pr1E11 monoclonal antibody reacts with human TROP-2, also known as TACSTD2, EGP-1, and GA733-1. TROP-2 is a type I transmembrane glycoprotein with high homology to TROP-1/EpCAM. TROP-2 spans the epithelial membrane surface and plays a role in embryonic development, cell self-renewal, proliferation, and transformation. TROP-2 is found on the surface of multiple normal epithelial tissues, including skin and oral mucosa. TROP-2 can promote tumor growth and its overexpression is common in many types of malignant epithelial tumors. A variety of human epithelial cancer cells are characterized by TROP-2 overexpression, including breast, lung, urothelial, gastric, colorectal, pancreatic, prostatic, cervical, head and neck, and ovarian carcinomas.

Specifications

Isotype Mouse IgG1, κ
Recommended Isotype Control(s) InVivoMAb mouse IgG1 isotype control, unknown specificity
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen Primary human prostate cancer cells
Reported Applications Western blot
Immunohistochemistry (frozen)
Flow cytometry
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <2EU/mg (<0.002EU/μg)
Determined by LAL gel clotting assay
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from tissue culture supernatant in an animal free facility
Purification Protein G
Molecular Weight 150 kDa
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.
Western Blot, Flow Cytometry, Immunohistochemistry (frozen)
Ikeda M, Yamaguchi M, Kato K, Nakamura K, Shiina S, Ichikawa-Ando T, Misaka H, Myojo K, Nakamura K, Sugimoto Y, Hamada H. (2015). "Pr1E11, a novel anti-TROP-2 antibody isolated by adenovirus-based antibody screening, recognizes a unique epitope" Biochem Biophys Res Commun 458(4):877-82. PubMed

TROP-2 is a type Ⅰ transmembrane glycoprotein that is highly expressed in various epithelial cancer cells, and its increased expression correlates with poor prognosis. Although several anti-TROP-2 antibodies have been described, they were found unsuitable for antitumor therapy use in vivo as naked antibodies. In this study, we established a novel anti-TROP-2 antibody, designated Pr1E11, from mice immunized with primary prostate cancer cells. Antibody screening was based on the infection activity of Adv-LacZ-FZ33, which displays an immunoglobulin G binding domain in the adenoviral fiber protein. We found that Pr1E11 specifically binds to TROP-2 with high affinity and recognizes diverse epithelial cancer cell lines and primary pancreatic cancer tissues. Epitope analysis using TROP-2 deletion mutants revealed that binding site of Pr1E11 is a cysteine-rich domain, a unique epitope compared with other available anti-TROP-2 antibodies. In addition, Pr1E11 exhibited low internalization activity, which may make it suitable for naked antibody therapeutics. Our results suggest that Pr1E11 may stimulate different biological activities from other anti-TROP-2 antibodies based on its unique binding epitope, and is a potential candidate for naked antibody therapeutics for various epithelial cancer treatments.