InVivoSIM anti-human IL-4Rα (CD124) (Dupilumab Biosimilar)
Catalog #SIM0023
Clone:
Dupilumab
Reactivities:
Human
Product Details
This non-therapeutic biosimilar antibody uses the same variable regions as the therapeutic antibody Dupilumab making it ideal for research use. This Dupilumab biosimilar reacts with human IL-4Rα. IL-4Rα also known as CD124, associates with either the common γ-chain (CD132) to form type 1 IL-4R which only binds IL-4 or with IL-13Rα1 to form type II IL-4R which can bind IL-4 or IL-13. IL-4Rα is expressed on various cell types, including T helper type 2 (Th2) cells, mast cells, basophils, eosinophils, macrophages, dendritic cells, and endothelial cells. The expression of IL-4Rα on these different cell types allows for a variety of cellular responses to IL-4 and IL-13, including regulation of immune cell differentiation, activation, proliferation, and survival. Additionally, IL-4 and IL-13 signaling through IL-4Rα can promote tissue repair and remodeling, as well as contribute to the pathogenesis of various allergic and inflammatory diseases. Dupilumab binds IL-4Rα and inhibits signaling of both IL-4 and IL-13. It has shown efficacy across multiple diseases with underlying type 2 signatures and is used for treatment of asthma, atopic dermatitis, and chronic sinusitis with nasal polyposis.Specifications
| Isotype | Human IgG4, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Human IL-4Rα |
| Reported Applications |
Functional assays |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<0.5EU/mg (<0.0005EU/μg) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
RecombiMAb human IgG4 (S228P) isotype control, anti-hen egg lysozyme
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer