InVivoMAb anti-mouse MHC Class I (H-2Kb/H-2Db)
Catalog #BE0468
Clone:
28-8-6S
Reactivities:
Mouse
Product Details
The 28-8-6S monoclonal antibody reacts with the mouse H-2Kb and H-2Db MHC Class I alloantigens. Murine MHC I Ī± chain is composed of H-2 with K, D, and L loci, thereby forming H-2K, H-2D, and H-2L genes, which are considered highly polymorphic because they carry mouse strain-specific haplotypes designated by a lower-case letter (e.g., a, b, d, k, q, s, etc.). At the protein level, the MHC Class I antigen is a cell surface receptor expressed by all nucleated cells at varying levels in mice of the H-2Kb and H-2Db haplotype, e.g., C57BL/6 mice. MHC I plays a central role in antigen presentation to T cells, thereby allowing the immune system to recognize and respond to foreign pathogens, infected cells, or self-antigens. MHC I is also involved in neuronal development, specifically in synaptic elimination or synaptic pruning. In experimental therapeutics, personalized cancer vaccines and other immunotherapies can be developed by taking advantage of the antigen specificity of the interaction between MHC I molecules, peptides, and TCR. The 28-8-6S monoclonal antibody has been extensively used to study the role of H-2Kb and H-2Db alloantigens. This antibody binds strongly with H-2Kb but has a relatively lower affinity for H-2Db. The 28-8-6S antibody can only recognize H-2Db if Ī²2m is present. This antibody does not cross-react with f, k, p, q, r, and s haplotypes.Specifications
| Isotype | Mouse IgG2a, Īŗ |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | C3H.SW mouse splenocytes |
| Reported Applications |
in vivo functional assays in vitro functional assays Complement-dependent cytotoxicity (CDC) Immunohistochemistry (frozen) Flow cytometry Immunopeptidomics Immunoprecipitation |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<2EU/mg (<0.002EU/Ī¼g) Determined by LAL gel clotting assay |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
InVivoMAb mouse IgG2a isotype control, unknown specificity
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer