Learn how other researchers have used the anti-mouse CD28 antibody.



InVivoMAb anti-mouse CD28

The D665 monoclonal antibody reacts with mouse CD28, a 45 kDa costimulatory receptor and a member of the Ig superfamily. CD28 is expressed by thymocytes, most peripheral T cells, and NK cells. CD28 is a receptor for CD80 (B7-1) and CD86 (B7-2). Signaling through CD28 induces IL-2 and IL-2 receptor expression and T cell proliferation. The D665 antibody is a CD28 superagonist and is most commonly used to induce the expansion of Treg cells in vivo in various mouse models of disease.

This Antibody Is Useful For:

  • in vivo T cell stimulation/activation
  • in vitro T cell stimulation/activation

As always Bio X Cell antibodies are specifically formulated for in vivo use and feature:

  • 95% purity
  • Ultra-low endotoxin levels
  • Preservative, stabilizer, and carrier protein-free


Recommended Control and Buffer:


InVivoMAb mouse IgG1 isotype control, unknown specificity


InVivoMAb mouse IgG2b isotype control, unknown specificity

The MOPC-21 monoclonal antibody is ideal for use as a non-reactive isotype-matched control for mouse IgG1 antibodies in most in vivo and in vitro applications




InVivoPure pH 7.0 Dilution Buffer

InVivoPure™ dilution buffers are specifically formulated and tested to satisfy the stringent requirements for in vivo applications. They are extremely low in endotoxin, have been screened for murine pathogens, tested in animal models for toxicity and are formulated with respect to buffer composition and pH to satisfy the requirements of Bio X Cell’s antibodies.