Catalog #BE0280FAB
InVivoMAb anti-mouse c-Kit (CD117) f(ab')2 fragments
Clone
2B8 f(ab')2 Fragments
Reactivities
Mouse
Isotype
Rat IgG2b, κ
Product Description
This product is an F(ab) fragment derived from the anti-mouse c-Kit antibody clone 2B8. The 2B8 monoclonal antibody reacts with mouse c-Kit, also known as CD117, Steel factor receptor, stem cell factor receptor, and mast cell growth factor receptor. c-Kit is a 145 kDa transmembrane receptor tyrosine kinase and member of the immunoglobulin superfamily. It is expressed on hematopoietic stem and progenitor cells, mast cells, and acute myeloid leukemia (AML) cells, where it serves as a critical receptor for the cytokine stem cell factor (SCF). Interaction between c-Kit and SCF promotes the proliferation, survival, and differentiation of hematopoietic progenitor cells. Unlike a full-length antibody, this F(ab) fragment contains only the antigen-binding region and lacks the Fc domain. As a result, the fragment retains specific binding to mouse c-Kit while eliminating Fc-mediated effector functions such as complement activation and Fcγ receptor engagement. Because the Fc region is absent, this format is useful in experiments where c-Kit binding, receptor engagement, or cell targeting is desired without Fc-dependent immune effects, including antibody-mediated cytotoxicity, Fcγ receptor–mediated cross-linking by accessory cells, or complement activation. The full-length 2B8 antibody has been studied extensively as a c-Kit-targeting reagent, including in antibody-drug conjugate approaches designed to selectively deplete hematopoietic stem cells. When conjugated to a cytotoxic compound, 2B8-based ADCs have been reported to potently deplete hematopoietic stem cells in vivo and enable donor hematopoietic cell engraftment without causing broad neutropenia or lymphopenia. Studies have also shown that 2B8-ADC is efficiently internalized and can deplete immunophenotypic and functional HSCs more effectively than some other murine anti-c-Kit clones. The F(ab) fragment format may provide a useful tool for studying c-Kit binding, receptor engagement, internalization, and targeted delivery while minimizing secondary Fc-mediated mechanisms that can complicate interpretation of results. This anti-mouse c-Kit F(ab) fragment is well suited for studies focused on c-Kit targeting, hematopoietic stem and progenitor cell biology, mast cell biology, AML research, and receptor-directed delivery approaches.
Specifications
| Isotype | Rat IgG2b, κ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Mouse bone marrow mast cells |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤1EU/mg (≤0.001EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE and SEC |
| Sterility | 0.2 µm filtration |
| Production | Pepsin Digest |
| Purification | Protein G |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |