RecombiMab anti-mouse c-Kit (CD117)

Catalog #CP070
Clone:
2B8-CP070
Reactivities:
Mouse

$541.00 - $7,325.00

$541.00 - $7.00

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  • 25 mg - $7,325.00
  • 5 mg - $2,097.00
  • 1 mg - $541.00
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In stock
Only %1 left
Isotype:
Mouse IgG2a, Īŗ
(switched from Rat IgG2b, Īŗ)

Product Details

The 2B8-CP070 monoclonal antibody is a recombinant, chimeric version of the original 2B8 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG2b, Īŗ to Mouse IgG2a, Īŗ for use in murine models. Species-matched chimeric antibodies exhibit regulated effector functions—including Fc receptor binding and complement activation—and result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.

The 2B8 monoclonal antibody reacts with mouse c-Kit also known as CD117, Steel factor receptor, stem cell factor receptor, and mast cell growth factor. c-Kit is a 145 kDa transmembrane tyrosine kinase and an immunoglobulin superfamily member. c-Kit is expressed on hematopoietic progenitor cells, mast cells, and acute myeloid leukemia (AML) cells and is a critical hematopoietic stem and progenitor cell receptor for the cytokine stem cell factor (SCF). The interaction of the c-Kit receptor and SCF promotes the proliferation and differentiation of hematopoietic progenitor cells. Studies have demonstrated that when 2B8 is conjugated to a cytotoxic compound, the antibody-drug conjugate (ADC) potently depleted greater than 99% of hematopoietic stem cells (HSCs) in-vivo and enabled the successful engraftment of donor hematopoietic cells without resulting in neutropenia or lymphopenia. The 2B8-ADC was more efficiently internalized by EML cells and more effective at depleting immunophenotypic and functional HSCs in vivo than murine anti-c-Kit clones 3C11 or ACK2.

Specifications

Isotype Mouse IgG2a
Recommended Isotype Control(s) RecombiMAb mouse IgG2a isotype control, unknown specificity
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Conjugation This product is unconjugated. Conjugation is available via our Antibody Conjugation Services.
Immunogen Mouse bone marrow mast cells
Reported Applications Western Blot
Flow Cytometry
Immunofluorescence
Immunohistochemistry
Antibody-Drug Conjugate
Reported for the original 2B8 antibody. For information on in vivo applications, please contact (technicalservice@bioxcell.com)
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
Aggregation <5%
Determined by SEC
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from mammalian cell supernatant in an animal-free facility
Purification Protein G
Molecular Weight 150 kDa
Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

See references for the original 2B8 clone: https://bioxcell.com/invivomab-anti-mouse-c-kit-cd117-be0280?