Catalog #CP070FAB
RecombiMAb anti-mouse c-kit F(ab) Fragment (HIS Sortase) - PREORDER
Clone
2B8-CP070FAB
Reactivities
Mouse
Isotype
Mouse IgG2a F(ab), k
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Product Description
2B8-CP070FAB is a 50 kDa recombinant F(ab) fragment derived from the anti-mouse c-Kit antibody CP070. The F(ab) fragment contains a monovalent antigen-binding domain of clone 2B8 with both 6xHIS and Sortase tags, but with no Fc region resulting in specific binding to mouse c-Kit while eliminating Fc-mediated effector functions. This format is useful in experiments where c-Kit binding, receptor engagement, or cell targeting is desired without Fc-dependent immune effects, including antibody-mediated cytotoxicity, Fcγ receptor–mediated cross-linking, or complement activation. 2B8-CP070FAB reacts with mouse c-Kit, also known as CD117, Steel factor receptor, stem cell factor receptor, and mast cell growth factor receptor. c-Kit is a 145 kDa transmembrane receptor tyrosine kinase and member of the immunoglobulin superfamily. It is expressed on hematopoietic stem and progenitor cells, mast cells, and acute myeloid leukemia (AML) cells, where it serves as a critical receptor for the cytokine stem cell factor (SCF). Interaction between c-Kit and SCF promotes the proliferation, survival, and differentiation of hematopoietic progenitor cells.
Specifications
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
|---|---|
| Mutations | F(ab) |
| Immunogen | Mouse bone marrow mast cells |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE and SEC |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Molecular Weight | 50 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |