Catalog #CP239
RecombiMAb anti-mouse c-Kit (CD117) (LALA-PG)
Clone
2B8-CP239
Reactivities
Mouse
Applications
Western Blot*
Flow Cytometry*
Immunofluorescence*
Immunohistochemistry*
Antibody-Drug Conjugate*
*Reported for the original 2B8 antibody. For information on in vivo applications, please contact (technicalservice@bioxcell.com)
Flow Cytometry*
Immunofluorescence*
Immunohistochemistry*
Antibody-Drug Conjugate*
*Reported for the original 2B8 antibody. For information on in vivo applications, please contact (technicalservice@bioxcell.com)
Isotype
Mouse IgG2a LALA-PG, κ
(switched from Rat IgG2b, κ)
(switched from Rat IgG2b, κ)
Product Description
The 2B8-CP239 monoclonal antibody is a recombinant, chimeric version of the original 2B8 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG2b, κ to Mouse IgG2a, κ for use in murine models. Additionally, the constant region includes the Fc silencing mutation LALA-PG, preventing Fc-dependent cellular depletion, phagocytosis, and complement activation. Species-matched chimeric antibodies support repeat and long-term dosing studies by reducing anti-drug antibody responses that can accelerate antibody clearance, compromise sustained target neutralization and confound interpretation of in vivo efficacy and safety findings. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
The 2B8 monoclonal antibody reacts with mouse c-Kit also known as CD117, Steel factor receptor, stem cell factor receptor, and mast cell growth factor. c-Kit is a 145 kDa transmembrane tyrosine kinase and an immunoglobulin superfamily member. c-Kit is expressed on hematopoietic progenitor cells, mast cells, and acute myeloid leukemia (AML) cells and is a critical hematopoietic stem and progenitor cell receptor for the cytokine stem cell factor (SCF). The interaction of the c-Kit receptor and SCF promotes the proliferation and differentiation of hematopoietic progenitor cells. Studies have demonstrated that when 2B8 is conjugated to a cytotoxic compound, the antibody-drug conjugate (ADC) potently depleted greater than 99% of hematopoietic stem cells (HSCs) in-vivo and enabled the successful engraftment of donor hematopoietic cells without resulting in neutropenia or lymphopenia. The 2B8-ADC was more efficiently internalized by EML cells and more effective at depleting immunophenotypic and functional HSCs in vivo than murine anti-c-Kit clones 3C11 or ACK2.
The 2B8 monoclonal antibody reacts with mouse c-Kit also known as CD117, Steel factor receptor, stem cell factor receptor, and mast cell growth factor. c-Kit is a 145 kDa transmembrane tyrosine kinase and an immunoglobulin superfamily member. c-Kit is expressed on hematopoietic progenitor cells, mast cells, and acute myeloid leukemia (AML) cells and is a critical hematopoietic stem and progenitor cell receptor for the cytokine stem cell factor (SCF). The interaction of the c-Kit receptor and SCF promotes the proliferation and differentiation of hematopoietic progenitor cells. Studies have demonstrated that when 2B8 is conjugated to a cytotoxic compound, the antibody-drug conjugate (ADC) potently depleted greater than 99% of hematopoietic stem cells (HSCs) in-vivo and enabled the successful engraftment of donor hematopoietic cells without resulting in neutropenia or lymphopenia. The 2B8-ADC was more efficiently internalized by EML cells and more effective at depleting immunophenotypic and functional HSCs in vivo than murine anti-c-Kit clones 3C11 or ACK2.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | LALA-PG |
| Immunogen | Mouse bone marrow mast cells |
| Reported Applications |
Western Blot* Flow Cytometry* Immunofluorescence* Immunohistochemistry* Antibody-Drug Conjugate* *Reported for the original 2B8 antibody. For information on in vivo applications, please contact (technicalservice@bioxcell.com) |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |