InVivoMAb anti-human EphA2

Catalog #BE0410
Clone:
SHM16
Reactivities:
Human

$164.00 - $4,280.00

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  • 100 mg - $4,280.00
  • 50 mg - $3,024.00
  • 25 mg - $2,009.00
  • 5 mg - $600.00
  • 1 mg - $164.00
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Product Details

The SHM16 monoclonal antibody reacts with EPHA2, a transmembrane receptor glycoprotein with widespread expression in epithelial cells. Owing to its cell type-specific expression, EphA2 was originally named epithelial cell kinase (eck), but subsequent experiments revealed its expression in many solid tumors, wherein its overexpression correlates with malignancy and a poor prognosis. Among its eight different interacting ephrin A-family ligands, EphA2 exhibits an overt preference for ephrin A1. Interestingly, ephrin A1’s expression pattern is generally observed to be attenuated in EphA2-overexpressing aggressive tumors. EphA2-ephrin A1 signaling regulates proliferation, survival, migration, morphology, cell-to-cell repulsion, and adhesion during embryonic development and controls the processes of angiogenesis and tumorigenesis. The SHM16 antibody, generated by immunizing mice with EphA2-positive human melanoma cells, is reported to interact with an EphA2 epitope differing from that affecting ephrin A1 binding to EphA2, and it doesn’t affect EphA2 interaction with ephrin A1 on the cell surface. The internalization of SHM16 through EphA2 has been shown to inhibit the proliferative and metastatic behavior of melanoma cells in vitro. An immunotoxin generated by conjugating the SHM16 antibody with saporin (an intracellular cytotoxin) is reported to exhibit dose-dependent growth inhibition and cytotoxicity in EphA2-positive melanoma cells in vitro, while the immunotoxin did not affect EphA2-negative cells. The SHM16 antibody has been used for characterization of human head and neck cancer cell lines using mass cytometry in Optimized Multicolor Immunofluorescence Panel-45, i.e., OMIP-45. Like most of the EphA-targeted monoclonal antibodies, SHM16 exhibits an agonist effect, and is reported to activate the EphA2 signaling pathway by mimicking the anti-oncogenic effect of ephrin-A1 on melanoma cell lines.

Specifications

Isotype Mouse IgG1, κ
Recommended Isotype Control(s) InVivoMAb mouse IgG1 isotype control, unknown specificity
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Conjugation This product is unconjugated. Conjugation is available via our Antibody Conjugation Services.
Immunogen Human melanoma cell line A375
Reported Applications in vitro stimulation of EphA2 signaling
Functional assays
Immunoprecipitation
Flow cytometry
Immunofluorescence
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <2EU/mg (<0.002EU/μg)
Determined by LAL gel clotting assay
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from cell culture supernatant in an animal-free facility
Purification Protein G
Molecular Weight 150 kDa
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.