Catalog #CP132
RecombiMAb anti-mouse VEGFR-2
Clone
DC101-CP132
Reactivities
Mouse
Isotype
Mouse IgG2a, κ
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Product Description
The DC101-CP132 monoclonal antibody is a chimeric version of the original DC101 antibody. The variable domain sequences are identical to the original DC101 but the constant region sequences have been switched from rat IgG1 to mouse IgG2a. The DC101-CP132 antibody contains no Fc mutations just as the original rat IgG1 antibody does not. The DC101-CP132 monoclonal antibody reacts with mouse VEGFR-2 (vascular endothelial growth factor receptor 2) also known as CD309, KDR, and Flk-1. VEGFR-2 is a member of the tyrosine protein kinase family. Upon binding to its ligand VEGF, VEGFR-2 pays key roles in vascular development and permeability. VEGFR-2 is expressed on endothelial cells at high levels in adult heart, lung, kidney, brain, and skeletal muscle as well as other tissues at lower levels. The DC101 antibody has been shown to inhibit VEGFR-2 signaling in vivo.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | InVivoPlus mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Mouse VEGFR-2-SEAPs soluble receptor |
| Reported Applications |
in vivo blocking of VEGF/VEGFR-2 signaling* in vitro blocking of VEGFR signaling* Western blot* *Reported for the original rat IgG1 DC101 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from CHO cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |