Catalog #CP157FAB
RecombiMAb anti-mouse PD-1 (CD279) F(ab) fragment
Clone
RMP1-14-CP157 f(ab) Fragments
Reactivities
Mouse
Isotype
Mouse IgG2a, κ
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Product Description
This product is a F(ab) fragment derived from the anti-mouse PD-1 antibody clone RMP1-14-CP157. PD-1, also known as CD279, is an inhibitory receptor expressed on activated T cells, B cells, and myeloid cells. Upon binding its ligands, PD-L1 and PD-L2, PD-1 negatively regulates immune-cell activation by reducing T cell receptor signaling, proliferation, cytokine production, and effector function. The RMP1-14 antibody has been reported to block PD-1/PD-L signaling. Unlike a full-length antibody, this F(ab) fragment contains only the antigen-binding region and lacks the Fc domain. As a result, the fragment retains specific binding to mouse PD-1 while eliminating Fc-mediated effector functions such as complement activation and Fcγ receptor engagement. This format is useful in experiments where PD-1 targeting or blockade is desired without Fc-mediated cross-linking, immune-cell depletion, or other Fc-dependent effects that could complicate interpretation of PD-1 biology. F(ab) fragments targeting PD-1 may also be useful in emerging targeted-delivery strategies. For example, the fragment can be used to coat nanoparticles or other delivery systems intended to target PD-1-expressing immune cells and deliver genetic, pharmacologic, or imaging payloads. By avoiding Fc-mediated immune interactions, F(ab) fragments can enable receptor-specific targeting while reducing the potential for unintended Fc-dependent modulation or elimination of PD-1-expressing cells. This anti-mouse PD-1 F(ab) fragment therefore provides a useful reagent for studies focused on PD-1 binding and blockade without Fc effects, mechanistic investigation of the PD-1 pathway, and targeted-delivery approaches.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Syrian Hamster BKH cells transfected with mouse PD-1 cDNA |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE and SEC |
| Sterility | 0.2 µm filtration |
| Production | Papain Digest |
| Purification | Protein G |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |