Name: RecombiMAb anti-mouse OX40 (CD134) (LALA-PG)
Brand: CP018
SKU: CP018
Availability: InStock

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Technical Data Sheet Safety Data Sheet CoA

Product Details

The OX86-CP018 monoclonal antibody is a chimeric version of the original OX-86 antibody. The variable domain sequences are identical to the original OX-86 but the constant region sequences have been switched from rat IgG1 to mouse IgG2a. The OX86-CP018 antibody also contains a LALA-PG mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors.. OX86-CP018 reacts with mouse OX-40 also known as CD134. OX-40 is a 50 kDa type I membrane glycoprotein and a member of the TNF receptor superfamily. OX-40 is expressed on activated CD4 and CD8 T cells, but is not found on resting naïve T cells or most resting memory T cells. Although it was originally thought that OX-40 expression was restricted to activated conventional T cells, it has now been visualized on activated regulatory T cells, NKT cells, NK cells, and neutrophils. OX-40 plays a major role in regulating both CD4 and CD8 T cell clonal expansion. It provides a costimulatory signal to an antigen-reacting naive T cells to prolong proliferation, as well as augment the production of several cytokines. This is demonstrated by OX-40 knockout mice which generate fewer primary effector CD4 T cells after immunization. Furthermore, in vivo treatment with an agonist antibody to OX-40 has been shown to strongly enhance the generation of antigen-specific effector T cells and prevent the induction of T cell tolerance. The OX-86 antibody is an agonistic antibody that has been shown to delay tumor growth in vivo.

Specifications

Isotype	Mouse IgG2a, κ
Recommended Isotype Control(s)	RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
Recommended Dilution Buffer	InVivoPure pH 7.0 Dilution Buffer
Conjugation	This product is unconjugated. Conjugation is available via our Antibody Conjugation Services.
Mutations	LALA-PG
Immunogen	Recombinant mouse OX40-CD4 chimeric protein
Reported Applications	in vivo OX40 activation* in vitro OX40 activation* Western blot* *Reported for the original rat IgG1 OX-86 antibody
Formulation	PBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin	<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
Aggregation	<5% Determined by SEC
Purity	>95% Determined by SDS-PAGE
Sterility	0.2 µm filtration
Production	Purified from HEK293 cell supernatant in an animal-free facility
Purification	Protein G
Molecular Weight	150 kDa
Murine Pathogen Tests	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
Storage	The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.