Name: RecombiMAb anti-mouse OX40 (CD134)
Brand: CP017
SKU: CP017
Availability: InStock

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Technical Data Sheet Safety Data Sheet CoA

Product Details

The OX86-CP017 monoclonal antibody is a chimeric version of the original OX-86 antibody. The variable domain sequences are identical to the original OX-86 but the constant region sequences have been switched from rat IgG1 to mouse IgG2a. The OX86-CP017 antibody contains no Fc mutations just as the original rat IgG1 antibody does not. OX86-CP017 reacts with mouse OX-40 also known as CD134. OX-40 is a 50 kDa type I membrane glycoprotein and a member of the TNF receptor superfamily. OX-40 is expressed on activated CD4 and CD8 T cells, but is not found on resting naïve T cells or most resting memory T cells. Although it was originally thought that OX-40 expression was restricted to activated conventional T cells, it has now been visualized on activated regulatory T cells, NKT cells, NK cells, and neutrophils. OX-40 plays a major role in regulating both CD4 and CD8 T cell clonal expansion. It provides a costimulatory signal to an antigen-reacting naive T cells to prolong proliferation, as well as augment the production of several cytokines. This is demonstrated by OX-40 knockout mice which generate fewer primary effector CD4 T cells after immunization. Furthermore, in vivo treatment with an agonist antibody to OX-40 has been shown to strongly enhance the generation of antigen-specific effector T cells and prevent the induction of T cell tolerance. The OX-86 antibody is an agonistic antibody that has been shown to delay tumor growth in vivo.

Specifications

Isotype	Mouse IgG2a, κ
Recommended Isotype Control(s)	InVivoPlus mouse IgG2a isotype control, unknown specificity
Recommended Dilution Buffer	InVivoPure pH 7.0 Dilution Buffer
Conjugation	This product is unconjugated. Conjugation is available via our Antibody Conjugation Services.
Immunogen	Recombinant mouse OX40-CD4 chimeric protein
Reported Applications	in vivo OX40 activation* in vitro OX40 activation* Western blot* *Reported for the original rat IgG1 OX-86 antibody
Formulation	PBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin	<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
Aggregation	<5% Determined by SEC
Purity	>95% Determined by SDS-PAGE
Sterility	0.2 µm filtration
Production	Purified from HEK293 cell supernatant in an animal-free facility
Purification	Protein G
Molecular Weight	150 kDa
Murine Pathogen Tests	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
Storage	The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.