RecombiMAb anti-mouse IFNAR-1

Catalog #CP056
Clone:
MAR1-5A3-CP056
Reactivities:
Mouse

$541.00 - $7,325.00

$541.00 - $7,325.00

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  • 25 mg - $7,325.00
  • 5 mg - $2,097.00
  • 1 mg - $541.00
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In stock
Only %1 left
Isotype:
Mouse IgG2a LALA-PG, kappa
(switched from mouse IgG1)

Product Details

MAR1-5A3-CP056 monoclonal antibody is a recombinant, chimeric version of the original MAR1-5A3 antibody. The variable domain sequences are identical to clone MAR1-5A3, but the constant region has been converted from mouse IgG1 to mouse IgG2a. MAR1-5A3-CP056 also contains Fc silencing mutations rendering it unable to bind to endogenous Fcγ receptors, similar to therapeutic anti-IFNAR-1 antibodies such as Anifrolumab. These mutations prevent Fc-effector functions like antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. MAR1-5A3-CP056 antibody reacts with mouse IFNAR-1 (IFN alpha/beta receptor subunit 1). IFNAR-1 is co-expressed with IFNAR-2 on most cell types and together these two subunits make up the heterodimeric Type I IFN Receptor complex. Type I IFNs (IFN-α/β) bind to the Type I IFN Receptor complex to induce cellular responses including induction of anti-viral, anti-microbial, anti-tumor, and autoimmune responses as well as to regulate activation, proliferation, and differentiation. The MAR1-5A3 antibody has demonstrated inhibition of Type I IFN receptor signaling in vitro and in vivo.

Specifications

Recommended Isotype Control(s) RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen Extracellular domain of mouse IFNAR-1
Reported Applications in vitro IFNAR-1 blockade*
in vivo IFNAR-1 blockade*
*Reported for the original mouse IgG1 antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
Aggregation <5%
Determined by SEC
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from HEK293 cell supernatant in an animal-free facility
Purification Protein G
Molecular Weight 150 kDa
Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.