Catalog #CP056
RecombiMAb anti-mouse IFNAR-1
Clone
MAR1-5A3-CP056
Isotype
Mouse IgG2a (LALA-PG), κ
(Switched from Mouse IgG1, κ)
(Switched from Mouse IgG1, κ)
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Product Description
The MAR1-5A3-CP056 monoclonal antibody is a recombinant, chimeric version of the original MAR1-5A3 antibody. The variable domain sequences are identical to clone MAR1-5A3, but the constant region has been converted from mouse IgG1 to mouse IgG2a. MAR1-5A3-CP056 also contains Fc silencing mutations rendering it unable to bind to endogenous Fcγ receptors like therapeutic anti-IFNAR-1 antibodies such as Anifrolumab. These mutations prevent Fc-effector functions such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
MAR1-5A3-CP056 antibody reacts with mouse IFNAR-1 (IFN alpha/beta receptor subunit 1). IFNAR-1 is co-expressed with IFNAR-2 on most cell types and together these two subunits make up the heterodimeric Type I IFN Receptor complex. Type I IFNs (IFN-α/β) bind to the Type I IFN Receptor complex to induce cellular responses including induction of anti-viral, anti-microbial, anti-tumor, and autoimmune responses as well as to regulate activation, proliferation, and differentiation. A growing body of evidence supports the roles of type I IFNs in the immunopathogenesis of autoimmune diseases such as systemic lupus erythematosus (SLE), Sjögren’s syndrome, myositis, scleroderma and rheumatoid arthritis and other interferonopathies. The MAR1-5A3 antibody has been demonstrated to inhibit Type I IFN receptor signaling in vitro and in vivo.
MAR1-5A3-CP056 antibody reacts with mouse IFNAR-1 (IFN alpha/beta receptor subunit 1). IFNAR-1 is co-expressed with IFNAR-2 on most cell types and together these two subunits make up the heterodimeric Type I IFN Receptor complex. Type I IFNs (IFN-α/β) bind to the Type I IFN Receptor complex to induce cellular responses including induction of anti-viral, anti-microbial, anti-tumor, and autoimmune responses as well as to regulate activation, proliferation, and differentiation. A growing body of evidence supports the roles of type I IFNs in the immunopathogenesis of autoimmune diseases such as systemic lupus erythematosus (SLE), Sjögren’s syndrome, myositis, scleroderma and rheumatoid arthritis and other interferonopathies. The MAR1-5A3 antibody has been demonstrated to inhibit Type I IFN receptor signaling in vitro and in vivo.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Extracellular domain of mouse IFNAR-1 |
| Reported Applications |
in vitro IFNAR-1 blockade* in vivo IFNAR-1 blockade* *Reported for the original mouse IgG1 antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |