RecombiMAb anti-mouse CSF1R (CD115)
Catalog #CP131
Clone:
AFS98-CP131
Reactivities:
Mouse
Isotype:
Mouse IgG2a
(switched from rat IgG2a)
(switched from rat IgG2a)
Product Details
The AFS98-CP131 monoclonal antibody is a chimeric version of the original AFS98 antibody. The variable domain sequences are identical to the original AFS98 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a. The AFS98-CP131 antibody contains no Fc mutations just as the original rat IgG2a antibody does not. The AFS98-CP131 monoclonal antibody reacts with mouse colony stimulating factor 1 receptor (CSF1R), also known as macrophage colony-stimulating factor receptor (M-CSFR), and CD115. CSF1R is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. In mice CSF1R is expressed by monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts. CSF1R is a receptor for CSF1 and CSF1 signaling through CSF1R regulates the proliferation and differentiation of cells in the monocytic lineage. The AFS98 antibody has been reported to deplete macrophages and block CSFR1 in vivo.Specifications
| Isotype | Mouse IgG2a, Īŗ |
|---|---|
| Recommended Isotype Control(s) | InVivoPlus mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Not available or unknown |
| Reported Applications |
in vivo macrophage depletion* in vitro CSF1R neutralization* in vivo monocyte depletion* Flow cytometry* Western blot* *Reported for the original rat IgG2a AFS98 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<1EU/mg (<0.001EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from CHO cell supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Additional Formats
Recommended Products
-
Recommended Isotype Control(s)
InVivoPlus mouse IgG2a isotype control, unknown specificity
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
See the references for the original rat IgG2a AFS98 antibody (https://bioxcell.com/catalogsearch/result/?q=BP0213).
