RecombiMAb anti-mouse CD16/CD32 (LALA-PG)

Catalog #CP026
Clone:
2.4G2-CP026
Reactivities:
Mouse

$515.00

$515.00

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Isotype:
Mouse IgG2a
(switched from rat IgG2b)

Product Details

The 2.4G2-CP026 monoclonal antibody is a chimeric version of the original 2.4G2 antibody. The variable domain sequences are identical to the original 2.4G2 but the constant region sequences have been switched from rat IgG2b to mouse IgG2a. The 2.4G2-CP026 antibody also contains a LALA-PG mutation in the Fc fragment rendering it unable to bind to endogenous FcĪ³ receptors. 2.4G2-CP026 reacts specifically with mouse CD16 (FcĪ³RIII) and CD32 (FcĪ³RII). It has also been reported to react non-specifically via its Fc domain to FcĪ³RI. CD16 and CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. These receptors bind to the Fc portion of antibody-antigen complexes and play a role in adaptive immune responses. The 2.4G2 antibody is commonly used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcĪ³III and FcĪ³II, and possibly FcĪ³I, receptors prior to staining with antigen specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptorsĀ in vivo.

Specifications

Isotype Mouse IgG2a,Ā Īŗ
Recommended Isotype Control(s) RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Conjugation This product is unconjugated. Conjugation is available via our Antibody Conjugation Services.
Mutations LALA-PG
Immunogen BALB/c mouse macrophage cell line J774
Reported Applications in vivo Fc receptor blocking*
Fc receptor blocking, flow cytometry*
Fc receptor blocking, immunofluorescence*
*Reported for the original rat IgG2b 2.4G2 antibody
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/Ī¼g)
Determined by LAL gel clotting assay
Aggregation <5%
Determined by SEC
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 Āµm filtration
Production Purified from HEK293 cell supernatant in an animal-free facility
Purification Protein G
Molecular Weight 150 kDa
Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theilerā€™s Murine Encephalomyelitis: Negative
Storage The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze.

See the references for the original rat IgG2b 2.4G2 antibody (https://bioxcell.com/catalogsearch/result/?q=BP0307).