RecombiMAb anti-mouse CD16/CD32 (LALA-PG)
Catalog #CP026
Clone:
2.4G2-CP026
Reactivities:
Mouse
Isotype:
Mouse IgG2a
(switched from rat IgG2b)
(switched from rat IgG2b)
Product Details
The 2.4G2-CP026 monoclonal antibody is a chimeric version of the original 2.4G2 antibody. The variable domain sequences are identical to the original 2.4G2 but the constant region sequences have been switched from rat IgG2b to mouse IgG2a. The 2.4G2-CP026 antibody also contains a LALA-PG mutation in the Fc fragment rendering it unable to bind to endogenous FcĪ³ receptors. 2.4G2-CP026 reacts specifically with mouse CD16 (FcĪ³RIII) and CD32 (FcĪ³RII). It has also been reported to react non-specifically via its Fc domain to FcĪ³RI. CD16 and CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. These receptors bind to the Fc portion of antibody-antigen complexes and play a role in adaptive immune responses. The 2.4G2 antibody is commonly used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcĪ³III and FcĪ³II, and possibly FcĪ³I, receptors prior to staining with antigen specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptorsĀ in vivo.Specifications
| Isotype | Mouse IgG2a,Ā Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Mutations | LALA-PG |
| Immunogen | BALB/c mouse macrophage cell line J774 |
| Reported Applications |
in vivo Fc receptor blocking* Fc receptor blocking, flow cytometry* Fc receptor blocking, immunofluorescence* *Reported for the original rat IgG2b 2.4G2 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<1EU/mg (<0.001EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Additional Formats
Recommended Products
-
Recommended Isotype Control(s)
RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
See the references for the original rat IgG2b 2.4G2 antibody (https://bioxcell.com/catalogsearch/result/?q=BP0307).
