RecombiMAb anti-mouse CCR8

Catalog #CP080
Clone:
C8Mab-2-CP080
Reactivities:
Mouse

$541.00 - $7,325.00

$541.00 - $7.00

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  • 25 mg - $7,325.00
  • 5 mg - $2,097.00
  • 1 mg - $541.00
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In stock
Only %1 left
Isotype:
Mouse IgG2a, kappa
(switched from Rat IgG2b, kappa)

Product Details

The C8Mab-2-CP080 monoclonal antibody is a recombinant, chimeric version of the original C8Mab-2 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG2b, Īŗ to Mouse IgG2a, Īŗ for use in murine models. Species-matched chimeric antibodies exhibit regulated effector functions—including Fc receptor binding and complement activation—and result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. The anti-tumor activity of anti-CCR8 antibodies has been demonstrated to require Fc-mediated effector function with studies confirming the superior mouse IgG2a antibody binding to mouse FcγRIII and mouse FcγRIV is crucial for NK cell-mediated ADCC and macrophage-mediated ADCP in mice. The highly controlled sequence and lack of genetic drift in recombinant antibodies also provides more reliable and reproducible results over hybridoma derived antibodies.

The C8Mab-2 monoclonal antibody recognizes the N-terminal region (1–33 amino acids) of mouse C-C chemokine receptor type 8 (CCR8), also known as CKR-8, CDw198, CMKBRL2, CMKBR8, and GPRCY6. CCR8 is a seven-pass transmembrane chemokine receptor and a member of the G protein-coupled receptor (GPCR) family. CCR8 ligands include CCL1, CCL16, and CCL8 (mCCL8) or CCL18 (hCCL18, a functional analog of mouse CCL8). Human and mouse CCR8 as well as its primary ligand CCL1 are structurally related, and this ligand is critical for skin homing of T cells and the survival of the regulatory T cells (Tregs) as well as their chemotaxis into tumors. CCR8 is predominantly expressed on activated Tregs marking the most suppressive and proliferative Treg population residing in the TME. Regulatory T cells (Tregs) are immunosuppressive cells essential for maintaining peripheral immune tolerance and preventing harmful autoimmune responses. A deficiency in their number or function can lead to the development of autoimmune disorders. Conversely, an abundance of Tregs, particularly a high Treg-to-CD8+ T effector cell ratio, can hinder anti-tumor immune surveillance and promote cancer progression. CCR8, a surface receptor selectively expressed on activated Tregs within tumors, has emerged as a promising therapeutic target. Its selective expression offers the potential to enhance anticancer responses while minimizing the safety risks associated with earlier systemic Treg-targeting strategies. Recent in-vivo studies have documented the involvement of CCR8 in type 2 inflammatory diseases, including atopic dermatitis (AD) and allergic enteritis (AE). In the tumor microenvironment, CCR8+ Treg numbers directly correlate with an advanced state of cancer, and therapeutic depletion of CCR8+ tumor-infiltrating Tregs (ti-Tregs) is shown to exert antitumor immunity and synergism with anti-PD-1 therapy.

Specifications

Isotype Mouse IgG2a, Īŗ
Recommended Isotype Control(s) RecombiMAb mouse IgG2a isotype control, unknown specificity
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Conjugation This product is unconjugated. Conjugation is available via our Antibody Conjugation Services.
Reported Applications Western blot
*Flow Cytometry
*Immunofluorescence
*Reported for the original C8Mab-2 antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
Aggregation <5%
Determined by SEC
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from mammalian cell supernatant in an animal-free facility
Purification Protein G
Molecular Weight 150 kDa
Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

Additional Formats

Please see references for the original Rat IgG2b C8Mab-2 antibody (https://bioxcell.com/invivomab-anti-mouse-ccr8-cdw198-be0464).