Catalog #BE0174FAB
InVivoMAb anti-mouse LAG-3 F(ab) fragment
Clone
C9B7W f(ab) Fragments
Reactivities
Mouse
Isotype
Rat IgG1, κ
Product Description
This product is an F(ab) fragment derived from the anti-mouse LAG-3 antibody clone C9B7W. The C9B7W monoclonal antibody reacts with mouse LAG-3, also known as CD223, a 70 kDa type I transmembrane glycoprotein encoded by the Lag3 gene and belonging to the immunoglobulin superfamily. LAG-3 is expressed by activated T lymphocytes, NK cells, and regulatory T cells. Its primary ligand is MHC class II, which LAG-3 binds with higher affinity than CD4. LAG-3 is generally associated with immune checkpoint activity and has been reported to downregulate TCR signaling, inhibit CD4-dependent T cell function, and contribute to the suppressive function of regulatory T cells. In some contexts, LAG-3 has also been reported to promote immune responses through activation of antigen-presenting cells. Unlike a full-length antibody, this F(ab) fragment contains only the antigen-binding region and lacks the Fc domain. As a result, the fragment retains specific binding to mouse LAG-3 while eliminating Fc-mediated effector functions such as complement activation and Fcγ receptor engagement. Because the Fc region is absent, this format is useful in experiments where LAG-3 binding or receptor engagement is desired without Fc-dependent immune effects, including antibody-mediated cytotoxicity, Fcγ receptor–mediated cross-linking by accessory cells, or complement activation. The full-length C9B7W antibody has been reported to block the function of murine LAG-3 in vivo and in vitro, although studies suggest that this antibody does not block LAG-3 binding to MHC class II. The F(ab) fragment format may therefore provide a useful tool for studying LAG-3 biology, receptor engagement, and immune checkpoint signaling while minimizing secondary Fc-mediated mechanisms that can complicate interpretation of results. This anti-mouse LAG-3 F(ab) fragment is well suited for studies focused on LAG-3 targeting, checkpoint receptor engagement without Fc-mediated effects, and the functional role of LAG-3 in T cell, NK cell, and regulatory T cell biology.
Specifications
| Isotype | Rat IgG1, κ |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Mouse CD223-Ig fusion protein |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤1EU/mg (≤0.001EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE and SEC |
| Sterility | 0.2 µm filtration |
| Production | Papain Digest |
| Purification | Protein G |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |