RecombiMAb anti-mouse LAG-3

Catalog #CP014
Clone:
C9B7W-CP014
Reactivities:
Mouse
Isotype:
Mouse IgG2a
(switched from rat IgG1)

$500.00

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Product Details

The C9B7W-CP014 monoclonal antibody is a chimeric version of the original C9B7W antibody. The variable domain sequences are identical to the original C9B7W but the constant region sequences have been switched from rat IgG1 to mouse IgG2a. The C9B7W-CP014 antibody contains no Fc mutations just as the original rat IgG1 antibody does not. C9B7W-CP014 reacts with mouse LAG-3 also known as CD223. LAG-3 is a 70 kDa type I transmembrane glycoprotein encoded by the Lag3 gene that belongs to the immunoglobulin superfamily. LAG-3 is expressed by activated T lymphocytes, NK cells, and T regulatory cells. LAG-3’s main ligand is MHC class II which it binds to with a higher affinity than even CD4 does. Upon binding LAG-3 is thought to play similar roles as CTLA-4 and PD-1 including downregulation of TCR signaling and inhibition of CD4-dependent T cell function. LAG-3 has also been demonstrated to contribute to the suppressor function of T regulatory cells. In contrast to inhibition, LAG-3 has been shown to promotes immune responses by activating antigen-presenting cells. The C9B7W antibody has been reported to block the function of murine LAG-3 in vivo and in vitro but studies suggest that the antibody does not block binding of LAG-3 to MHC class II.

Specifications

Isotype Mouse IgG2a, κ
Recommended Isotype Control(s) InVivoPlus mouse IgG2a isotype control, unknown specificity
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen Mouse CD223-Ig fusion protein
Reported Applications in vivo LAG-3 neutralization*
in vitro LAG-3 neutralization*
Flow cytometry*
Western blot*
*Reported for the original rat IgG1 C9B7W antibody
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
Aggregation <5%
Determined by SEC
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from HEK293 cell supernatant in an animal free facility
Purification Protein G
Molecular Weight 150 kDa
Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

See the references for the original rat IgG1 C9B7W antibody (https://bioxcell.com/catalogsearch/result/?q=BP0174).