InVivoMAb anti-mouse MHC Class II (βchain)
Product Details
The KL277 monoclonal antibody reacts with mouse MHC Class II haplotypes I-Ab, I-Ad, I-Ap, I-Aq, I-Abu, and I-Abw. The antibody does not react with I-Aa, I-Ak, I-Af, I-Aj, I-As, or I-Atl haplotypes.Specifications
| Isotype | Hamster/Mouse IgG |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Synthetic peptide corresponding to residues 146-177 of the mouse Ab/beta protein |
| Reported Applications | Western blot |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein G |
| RRID | AB_10951148 |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
Recommended Products
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
Western Blot
Sofron, A., et al. (2015). "High-resolution analysis of the murine MHC class II immunopeptidome" Eur J Immunol. doi : 10.1002/eji.201545930. PubMed
The reliable identification of peptides bound to MHC class II is fundamental for the study of the host immune response against pathogens and the pathogenesis of autoimmune conditions. Here we describe an improved methodology combining immuno-affinity enrichment of MHC class II complexes, optimized elution conditions and quadrupole Orbitrap mass spectrometry-based characterization of the immunopeptidome. The methodology allowed the identification of over 1000 peptides with 1% false discovery rate from 108 murine A20 lymphoma cells. The study revealed the I-Ad specific motif in high resolution after multi-sequence alignment. The methodology was generally applied to the purification of MHC class II from cell lines and murine spleens. We identified 2963 peptides from BALB/c and 2712 from C57BL/6 mouse spleens. The identification of peptides that bound to MHC class II in vitro and in vivo will facilitate the characterization of T-cell specificities, as well as the development of biotherapeutics and vaccines. This article is protected by copyright. All rights reserved.
- WB,
- Mus musculus (House mouse),
- Immunology and Microbiology
High-resolution analysis of the murine MHC class II immunopeptidome.
In European Journal of Immunology on 1 February 2016 by Sofron, A., Ritz, D., et al.
PubMed
The reliable identification of peptides bound to major histocompatibility complex (MHC) class II is fundamental for the study of the host immune response against pathogens and the pathogenesis of autoimmune conditions. Here, we describe an improved methodology combining immuno-affinity enrichment of MHC class II complexes, optimized elution conditions and quadrupole Orbitrap mass spectrometry-based characterization of the immunopeptidome. The methodology allowed the identification of over 1000 peptides with 1% false discovery rate from 10(8) murine A20 lymphoma cells. The study revealed the I-A(d) -specific motif in high resolution after multisequence alignment. The methodology was generally applied to the purification of MHC class II from cell lines and murine spleens. We identified 2963 peptides from BALB/c and 2712 from C57BL/6 mouse spleens. The identification of peptides bound to MHC class II in vitro and in vivo will facilitate the characterization of T-cell specificities, as well as the development of biotherapeutics and vaccines.© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
