InVivoMAb anti-mouse CD3ε F(ab')2 fragment

Catalog #BE0001-1FAB
Product Citations:
13
Clone:
145-2C11 f(ab')2 Fragments
Reactivities:
Mouse

$1,061.00 - $4,386.00

$1,061.00 - $4,386.00

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  • 50 mg - $4,386.00
  • 25 mg - $3,125.00
  • 10 mg - $1,864.00
  • 5 mg - $1,061.00
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In stock

Product Details

The anti-mouse CD3 F(ab’)2 fragment is the antigen binding F(ab’)2 fragments of the 145-2C11 monoclonal antibody only. The majority of the Fc fragment has been removed via pepsin digestion. The 145-2C11 antibody F(ab’)2 fragment reacts with mouse CD3ε, a 20 kDa transmembrane cell-surface protein that belongs to the immunoglobulin superfamily. CD3ε is one of five polypeptide chains that combine to form the TCR complex. CD3ε is expressed on T lymphocytes, NK-T cells, and to varying degrees on developing thymocytes. CD3 plays roles in TCR signaling, T lymphocyte activation, and antigen recognition. The complete 145-2C11 antibody is commonly used to stimulate T cells in vitro however, when used in vivo activation of resting T cells results in cytokine release and toxicity caused by Ab-mediated cross-linking of T cells and Fcγ receptor-bearing cells. To avoid these complications non-Fc receptor binding F(ab’)2 fragments of the 145-2C11 antibody are commonly used. This non-FcR-binding anti-CD3 induces apoptosis of Ag-activated T cells in vivo by allowing durable expression of the TCR and sustained signaling. Foxp3+ Tregs have been shown to be resistant to CD3 antibody-mediated depletion.

Specifications

Isotype Armenian Hamster IgG1
Recommended Isotype Control(s) InVivoMAb hamster IgG f(ab')2 fragments
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen Mouse BM10-37 cytotoxic T cells
Reported Applications in vivo T cell depletion
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <2EU/mg (<0.002EU/μg)
Determined by LAL gel clotting assay
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 μM filtered
Production Pepsin Digest
Purification Protein A
RRID AB_2687679
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.