Catalog #CPB536
Trispecific anti-mouse PD-L1× anti-mouse TIGIT× anti-mouse LAG3 - PREORDER
Clone
derived from clones 1G9, C9B7W, 10F.9G2
Reactivities
Mouse
Applications
ELISA,
For information on in vivo applications, please contact technicalservice@bioxcell.com
For information on in vivo applications, please contact technicalservice@bioxcell.com
Isotype
Mouse IgG2a-scFv, κ
Product Description
CPB536 is a 2+2 tetravalent trispecific designed to simultaneously target mouse programmed cell death 1 ligand 1 (PD-L1, CD274, B7-H1), mouse T cell immunoreceptor with Ig and ITIM domains (TIGIT) and mouse lymphocyte-activation gene 3 (LAG3). CPB536 features monovalent binding to mouse TIGIT and mouse LAG3, with bivalent binding to mouse PD-L1. CPB536 contains a murine IgG2a constant region to reduce immunogenicity and the formation of anti-drug antibodies (ADAs) in mouse models. This trispecific was designed to bind antigens on different cells, bridging tumor/APCs with T cells. PD-L1 blockade prevents PD-1/PD-L1 suppression and provides tumor/APC anchoring, TIGIT blockade relieves TIGIT-mediated inhibitory signaling on dysfunctional T cells and LAG-3 blockade relieves a second major exhaustion pathway, improving T-cell activation and expansion. Fc-competent PD-L1 × TIGIT × LAG-3 trispecifics simultaneously block three inhibitory axes to promote tumor-localized T-cell engagement, leading to stronger anti-tumor T-cell responses.
Specifications
| Isotype | Mouse IgG2a-scFv, κ |
|---|---|
| Recommended Isotype Control(s) | Bispecific mouse scFv-IgG2a, kappa isotype control |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Reported Applications |
ELISA, For information on in vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein A |
| RRID | AB_3751716 |
| Molecular Weight | 200.5 |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |