RecombiMAb anti-mouse VISTA
Catalog #CP053
Clone:
13F3-CP053
Reactivities:
Mouse
Isotype:
Mouse IgG2a
(switched from Armenian hamster IgG)
(switched from Armenian hamster IgG)
Product Details
The 13F3-CP053 monoclonal antibody is a chimeric version of the original 13F3 antibody. The variable domain sequences are identical to the original 13F3 but the constant region sequences have been switched from Armenian hamster IgG to mouse IgG2a. The 13F3-CP053 antibody contains no Fc mutations just as the original hamster IgG antibody does not. The 13F3-CP053 monoclonal antibody reacts with mouse V-domain Ig suppressor of T cell activation (VISTA) also known as PD-1H and B7-H5. VISTA is a 309 aa type I transmembrane glycoprotein and a member of the Ig superfamily. VISTA is expressed on naĆÆve and activated T cells, NK cells, macrophages, dendritic cells, and neutrophils. VISTA functions as a negative immune-checkpoint protein that suppresses T cell cytokine production and proliferation. VISTA is overexpressed by tumor-infiltrating lymphocytes, such as myeloid cells and regulatory T cells. Blockade of VISTA with the original 13F3 antibody results in delayed tumor growth in mouse models of melanoma.Specifications
| Isotype | Mouse IgG2a, Īŗ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | EL4 cells overexpressing mouse VISTA-RFP and then boosted with VISTA-Ig fusion protein |
| Reported Applications |
in vivo blocking of VISTA signaling* in vitro blocking of VISTA signaling* Flow cytometry* *Reported for the original Armenian hamster IgG 13F3 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<1EU/mg (<0.001EU/Ī¼g) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
>95% Determined by SDS-PAGE |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theilerās Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Additional Formats
Recommended Products
-
Recommended Isotype Control(s)
RecombiMAb mouse IgG2a isotype control, anti-hen egg lysozyme
-
Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
See the references for the original Armenian hamster 13F3 antibody (https://bioxcell.com/bp0310).