Catalog #CP066
RecombiMAb anti-mouse OX40L (CD252, CD134L)
Clone
RM134L-CP066
Reactivities
Mouse
Isotype
Mouse IgG2a, κ
(converted from Rat IgG2b, κ)
(converted from Rat IgG2b, κ)
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Product Description
The RM134L-CP066 monoclonal antibody is a recombinant, murine chimeric version of the original RM134L antibody. The variable domain sequences are identical but the constant region sequences have been switched from rat IgG2b, κ to mouse IgG2a, κ for use in murine models. Species-matched chimeric antibodies exhibit regulated effector functions—including Fc receptor binding and complement activation—and cause less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies. The RM134L monoclonal antibody reacts with mouse OX-40L (also known CD252, CD134L, TNFSF4). OX-40L is a 35 kDa member of the TNF superfamily that is expressed on activated B cells, dendritic cells, vascular endothelial cells, mast cells, and natural killer cells. OX40L is the ligand for OX-40 (CD134). OX-40 signaling regulates both CD4 and CD8 T cell clonal expansion, provides a costimulatory signal to an antigen-reacting naive T cells prolonging proliferation, augments the production of several cytokines including RANTES, IL-2, IL-3, and IFN-g and plays an important role in immunotolerance. In vivo treatment with the RM134L antibody has been shown to block the interaction between OX40L and OX40.
Specifications
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a isotype control, unknown specificity |
|---|---|
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Rat NRK-52E cells transfected with mouse OX40L |
| Reported Applications |
Flow Cytometry in vitro OX40L neutralization in vivo blocking of OX40/OX40L signaling *Reported for the original rat IgG2b RM134L antibody. For information on in vivo applications, please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |