Name: RecombiMAb anti-mouse ICOSL (CD275) (D265A)
Brand: CP044
SKU: CP044
Availability: InStock

Skip to the beginning of the images gallery

Technical Data Sheet Safety Data Sheet CoA

Product Details

The HK5.3-CP044 monoclonal antibody is a chimeric version of the original HK5.3 antibody. The variable domain sequences are identical to the original HK5.3 but the constant region sequences have been switched from rat IgG2a to mouse IgG1. The HK5.3-CP044 antibody also contains a D265A mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. The HK5.3-CP044 monoclonal antibody reacts with mouse ICOSL (inducible T cell co-stimulator ligand) also known as CD275, B7RP-1, and B7-H2. ICOSL is a 40 kDa immune checkpoint protein belonging to the Ig receptor superfamily. Upon ICOSL binding, ICOS signaling co-stimulates T and B cell responses. The ligand Is expressed on antigen presenting cells including splenic B cells, dendritic cells, and macrophages. ICOS signaling is also thought to be important for maintaining regulatory T cell homeostasis. The HK5.3 antibody has been shown to block the binding of ICOSL to ICOS both in vitro and in vivo. HK5.3 treatment of mice has been reported to lead to a loss of regulatory T cells.

Specifications

Isotype	Mouse IgG1, κ
Recommended Isotype Control(s)	RecombiMAb mouse IgG1 (D265A) isotype control, anti-hen egg lysozyme
Recommended Dilution Buffer	InVivoPure pH 7.0 Dilution Buffer
Conjugation	This product is unconjugated. Conjugation is available via our Antibody Conjugation Services.
Mutations	D265A
Immunogen	Mouse B7-RP1 transfected cell line
Reported Applications	in vivo ICOSL neutralization* *Reported for the original rat IgG2a HK5.3 antibody
Formulation	PBS, pH 7.0 Contains no stabilizers or preservatives
Endotoxin	<1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay
Aggregation	<5% Determined by SEC
Purity	>95% Determined by SDS-PAGE
Sterility	0.2 µm filtration
Production	Purified from HEK293 cell supernatant in an animal-free facility
Purification	Protein G
Molecular Weight	150 kDa
Murine Pathogen Tests	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
Storage	The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.