RecombiMAb anti-mouse ICOSL (CD275) (D265A)

Catalog #CP044
Clone:
HK5.3-CP044
Reactivities:
Mouse
Isotype:
Mouse IgG1
(switched from rat IgG2a)

$500.00

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Product Details

The HK5.3-CP044 monoclonal antibody is a chimeric version of the original HK5.3 antibody. The variable domain sequences are identical to the original HK5.3 but the constant region sequences have been switched from rat IgG2a to mouse IgG1. The HK5.3-CP044 antibody also contains a D265A mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. The HK5.3-CP044 monoclonal antibody reacts with mouse ICOSL (inducible T cell co-stimulator ligand) also known as CD275, B7RP-1, and B7-H2. ICOSL is a 40 kDa immune checkpoint protein belonging to the Ig receptor superfamily. Upon ICOSL binding, ICOS signaling co-stimulates T and B cell responses. The ligand Is expressed on antigen presenting cells including splenic B cells, dendritic cells, and macrophages. ICOS signaling is also thought to be important for maintaining regulatory T cell homeostasis. The HK5.3 antibody has been shown to block the binding of ICOSL to ICOS both in vitro and in vivo. HK5.3 treatment of mice has been reported to lead to a loss of regulatory T cells.

Specifications

Isotype Mouse IgG1, κ
Recommended Isotype Control(s) RecombiMAb mouse IgG1 (D265A) isotype control, anti-hen egg lysozyme
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Mutations D265A
Immunogen Mouse B7-RP1 transfected cell line
Reported Applications in vivo ICOSL neutralization*
*Reported for the original rat IgG2a HK5.3 antibody
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
Aggregation <5%
Determined by SEC
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from HEK293 cell supernatant in an animal free facility
Purification Protein G
Molecular Weight 150 kDa
Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

See the references for the original rat IgG2a HK5.3 antibody (https://bioxcell.com/catalogsearch/result/?q=BE0028).