Catalog #CP223
RecombiMAb anti-mouse/human TYRP1/TRP1 (gp75)
Clone
TA99-CP223
Reactivities
Mouse, Human
Applications
in vivo triggering of FcγRs*
Immunofluorescence*
Flow cytometry*
*Reported for the original TA99 antibody
Immunofluorescence*
Flow cytometry*
*Reported for the original TA99 antibody
Isotype
Mouse IgG2a, κ
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Product Description
The TA99-CP223 recombinant monoclonal antibody has an effector function competent Fc domain allowing for activation of Fcγ receptors (FcγRs) to trigger antibody‑dependent cellular cytotoxicity (ADCC), antibody‑dependent cellular phagocytosis (ADCP), complement‑dependent cytotoxicity (CDC) and opsonization to promote target cell depletion. The mouse IgG2a isotype demonstrates strong effector functions due to potent interaction with mFcγRIV, which is functionally similar to the FcγRIIIa receptor involved in human ADCC. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
The TA99-CP223 recombinant antibody reacts with human and mouse tyrosinase-related protein 1 (TYRP1) also known as glycoprotein 75 (gp75) and MEL-5. TYRP1 is a melanocyte-specific enzyme involved in melanin synthesis as well as the proliferation and cell death of melanocytes. TYRP1 is critical for skin, eye, and hair pigmentation. Overexpression of TYRP1 on melanoma cells makes TYRP1 an attractive target for antibody therapy in melanoma treatment.
The TA99-CP223 recombinant antibody reacts with human and mouse tyrosinase-related protein 1 (TYRP1) also known as glycoprotein 75 (gp75) and MEL-5. TYRP1 is a melanocyte-specific enzyme involved in melanin synthesis as well as the proliferation and cell death of melanocytes. TYRP1 is critical for skin, eye, and hair pigmentation. Overexpression of TYRP1 on melanoma cells makes TYRP1 an attractive target for antibody therapy in melanoma treatment.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | SK-MEL-23 melanoma cells |
| Reported Applications |
in vivo triggering of FcγRs* Immunofluorescence* Flow cytometry* *Reported for the original TA99 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein A |
| RRID | AB_3751693 |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |