Catalog #CP103
RecombiMAb anti-mouse CXCR4 (CD184)
Clone
Cx4Mab-1-CP103
Reactivities
Mouse
Isotype
Mouse IgG2a, κ
(switched from Rat Ig2a, κ)
(switched from Rat Ig2a, κ)
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Product Description
The Cx4Mab-1-CP103 monoclonal antibody is a recombinant, Fc-engineered chimeric version of the original Cx4Mab-1 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG2a, κ to Mouse IgG2a, κ for use in murine models. Species-matched chimeric antibodies exhibit regulated effector functions—including Fc receptor binding and complement activation—and result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. This antibody has an effector function competent Fc domain allowing for activation of Fcγ receptors (FcγRs) to trigger antibody‑dependent cellular cytotoxicity (ADCC), antibody‑dependent cellular phagocytosis (ADCP), complement‑dependent cytotoxicity (CDC) and opsonization to promote target cell depletion. The mouse IgG2a isotype demonstrates strong effector functions due to potent interaction with mFcγRIV, which is functionally similar to the FcγRIIIa receptor involved in human ADCC. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
The Cx4Mab-1 monoclonal antibody reacts with mouse CXC chemokine receptor 4 (CXCR4), also called fusin and CD184. CXCR4 is a hepta-transmembrane domain G protein-coupled receptor (GPCR) that is expressed in hematopoietic cells, endothelial cells, neurons, and embryonic as well as adult stem cells. The primary ligand of CXCR4 is CXCL12, also known as stromal cell-derived factor-1α (SDF-1α). The CXCR4-CXCL12 signaling pathway plays a critical role in activating multiple signaling pathways including ERK1/2, ras, p38 MAPK, PLC/MAPK, and SAPK/JNK. CXCR4-CXCL12 signaling also regulates several biological processes including cell survival, proliferation, migration, and stemness. Overexpression of CXCR4 is often linked to poor prognosis in various cancers, and during cancer metastasis, CXCR4 positive cancer cells are chemotactically homed to tissues such as the liver, bone marrow, lungs, and lymph nodes that express abundant levels of CXCL12. CXCR4 as well as CXCR4/CXCL12 antagonists are emerging as attractive targets for experimental cancer therapeutics.
The Cx4Mab-1 monoclonal antibody reacts with mouse CXC chemokine receptor 4 (CXCR4), also called fusin and CD184. CXCR4 is a hepta-transmembrane domain G protein-coupled receptor (GPCR) that is expressed in hematopoietic cells, endothelial cells, neurons, and embryonic as well as adult stem cells. The primary ligand of CXCR4 is CXCL12, also known as stromal cell-derived factor-1α (SDF-1α). The CXCR4-CXCL12 signaling pathway plays a critical role in activating multiple signaling pathways including ERK1/2, ras, p38 MAPK, PLC/MAPK, and SAPK/JNK. CXCR4-CXCL12 signaling also regulates several biological processes including cell survival, proliferation, migration, and stemness. Overexpression of CXCR4 is often linked to poor prognosis in various cancers, and during cancer metastasis, CXCR4 positive cancer cells are chemotactically homed to tissues such as the liver, bone marrow, lungs, and lymph nodes that express abundant levels of CXCL12. CXCR4 as well as CXCR4/CXCL12 antagonists are emerging as attractive targets for experimental cancer therapeutics.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | mCXCR4-PA tag overexpressing LN229 cells |
| Reported Applications |
Flow cytometry For details on in vivo applications please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |