Catalog #CP116
RecombiMAb anti-mouse CXCR3 (CD183)
Clone
Cx3Mab-4-CP116
Reactivities
Mouse
Isotype
Mouse IgG2a, κ
(switched from Rat IgG1, κ)
(switched from Rat IgG1, κ)
Product Description
The Cx3Mab-4-CP116 monoclonal antibody is a recombinant, Fc-engineered chimeric version of the original Cx3MAB-4 antibody. The variable domain sequences are identical but the constant region sequences have been switched from Rat IgG1, κ to Mouse IgG2a, κ for use in murine models. Species-matched chimeric antibodies exhibit regulated effector functions—including Fc receptor binding and complement activation—and result in less immunogenicity and formation of anti-drug antibodies (ADAs) than xenogenic antibodies in animal models. Cx3Mab-4-CP116 has an effector function competent Fc domain allowing for activation of Fcγ receptors (FcγRs) to trigger antibody‑dependent cellular cytotoxicity (ADCC), antibody‑dependent cellular phagocytosis (ADCP), complement‑dependent cytotoxicity (CDC) and opsonization facilitating target cell depletion. The mouse IgG2a isotype demonstrates strong effector functions due to potent interaction with mFcγRIV, which is functionally similar to the FcγRIIIa receptor involved in human ADCC. The highly controlled sequence and lack of genetic drift in recombinant antibodies provide more reliable and reproducible results over hybridoma derived antibodies.
Mouse CXCR3 (CD183) is a seven‑transmembrane G protein–coupled chemokine receptor expressed mainly on activated T cells, NK cells, and some other immune and stromal populations in mice. It binds the interferon-inducible CXC chemokines CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC), triggering G protein–dependent calcium flux, integrin activation, cytoskeletal rearrangement, and chemotactic migration toward inflamed tissues. CXCR3 is particularly enriched on Th1-polarized and effector/memory T cells, where it supports trafficking to sites of type 1 inflammation and contributes to processes such as allograft rejection and other T cell–mediated immune responses in mouse models.
Mouse CXCR3 (CD183) is a seven‑transmembrane G protein–coupled chemokine receptor expressed mainly on activated T cells, NK cells, and some other immune and stromal populations in mice. It binds the interferon-inducible CXC chemokines CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC), triggering G protein–dependent calcium flux, integrin activation, cytoskeletal rearrangement, and chemotactic migration toward inflamed tissues. CXCR3 is particularly enriched on Th1-polarized and effector/memory T cells, where it supports trafficking to sites of type 1 inflammation and contributes to processes such as allograft rejection and other T cell–mediated immune responses in mouse models.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | mouse CXCR3 overexpressing CHO cells |
| Reported Applications |
Flow Cytometry For details on in vivo applications please contact technicalservice@bioxcell.com |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |