Catalog #CP236
RecombiMAb anti-mouse CD8α (LALA-PG)
Clone
YTS 105.18-CP236
Reactivities
Mouse
Applications
in vivo functional assay*
in vitro functional assay*
in vivo blocking of CD8*
in vivo CAR-T generation via antibody-conjugated tLNP mRNA delivery
ELISA*
*Reported for the original Rat IgG2a, κ YTS 105.18 antibody
in vitro functional assay*
in vivo blocking of CD8*
in vivo CAR-T generation via antibody-conjugated tLNP mRNA delivery
ELISA*
*Reported for the original Rat IgG2a, κ YTS 105.18 antibody
Isotype
Mouse IgG2a (LALA-PG), κ
(switched from Rat IgG2a, κ)
(switched from Rat IgG2a, κ)
Product Description
The YTS 105.18 monoclonal antibody (also referred to as YTS105) reacts with mouse CD8α. CD8 is a transmembrane glycoprotein that functions as a co-receptor for the T-cell receptor (TCR) by binding peptide–MHC class I complexes. It is expressed predominantly on cytotoxic T cells and is also present on subsets of thymocytes, natural killer cells, and dendritic cells. CD8 commonly exists as a CD8αβ heterodimer but can also be expressed as a CD8αα homodimer. Both the CD8α and CD8β chains share significant homology to immunoglobulin variable light chains. The YTS 105.18 antibody recognizes a non-polymorphic epitope on murine CD8α, and it is reported to block CD8 co-receptor interactions with pMHC-I and TL (e.g., MHC-I–dependent CD8 T-cell responses). The non-depleting anti-CD8α YTS105 is shown to synergize with YTS177 and other antibodies in NOD mice by functionally restraining pathogenic CD8+ T cells, supporting combinatorial tolerance strategies across autoimmune and inflammatory models. This recombinant mouse IgG2a LALA-PG variant retains the YTS 105.18 antigen-binding variable regions while incorporating Fc-silencing mutations engineered to reduce Fcγ receptor- and C1q-mediated effector functions. This antibody is useful for Fc-silenced clean CD8 co-receptor functional modulation, CAR T biodistribution/phenotyping, and for CD8-targeted in vivo CAR-T generation via antibody-conjugated tLNP mRNA delivery.
Specifications
| Isotype | Mouse IgG2a (LALA-PG), κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Mutations | LALA-PG |
| Immunogen | Ly-2 transfected mouse L cells |
| Reported Applications |
in vivo functional assay* in vitro functional assay* in vivo blocking of CD8* in vivo CAR-T generation via antibody-conjugated tLNP mRNA delivery ELISA* *Reported for the original Rat IgG2a, κ YTS 105.18 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL assay |
| Aggregation |
<5% Determined by SEC |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from mammalian cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Murine Pathogen Tests |
Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |