RecombiMAb anti-mouse CD40L (CD154)

Catalog #CP033
Clone:
MR-1-CP033
Reactivities:
Mouse
Isotype:
Mouse IgG2a
(switched from Armenian hamster IgG)

$500.00

Choose an Option...
  • 1 mg - $500.00
  • Custom Amount (Quotes Only)
In stock
Only %1 left

Product Details

The MR-1-CP033 monoclonal antibody is a chimeric version of the original MR-1 antibody. The variable domain sequences are identical to the original MR-1 but the constant region sequences have been switched from Armenian hamster IgG to mouse IgG2a. The MR-1-CP033 antibody contains no Fc mutations just as the original Armenian hamster IgG antibody does not. The MR-1-CP033 antibody reacts with mouse CD154 also known as CD40 ligand. CD154 exists as a 39 kDa accessory molecule and belongs to the TNF superfamily of cytokines. CD154 is primarily expressed on the surface of activated CD4+ T lymphocytes but can also be expressed by platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, CD8+ T lymphocytes as well as non-hematopoietic cells including smooth muscle cells, endothelial cells, and epithelial cells. CD154 signals through CD40 and is thought to play a key role in T and B lymphocyte costimulation. The MR-1 monoclonal antibody has been reported to inhibit in vitro activation of B lymphocytes by blocking the binding of CD154 with CD40 on T helper cells as well as inhibit the formation of germinal centers and disrupt antigen-specific T cell responses. Additionally, the MR-1 antibody blocks interactions of T cells and antigen-presenting cells in vitro and blocks the development of experimental autoimmune disease in vivo.

Specifications

Isotype Mouse IgG2a, κ
Recommended Isotype Control(s) InVivoPlus mouse IgG2a isotype control, unknown specificity
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen Activated mouse Th1 clone D1.6
Reported Applications in vivo blocking of CD40/CD40L signaling*
in vitro blocking of CD40/CD40L signaling*
Western blot*
*Reported for the original Armenian hamster IgG MR-1 antibody
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/μg)
Determined by LAL gel clotting assay
Aggregation <5%
Determined by SEC
Purity >95%
Determined by SDS-PAGE
Sterility 0.2 µm filtration
Production Purified from HEK293 cell supernatant in an animal free facility
Purification Protein G
Molecular Weight 150 kDa
Murine Pathogen Tests Ectromelia/Mousepox Virus: Negative
Hantavirus: Negative
K Virus: Negative
Lactate Dehydrogenase-Elevating Virus: Negative
Lymphocytic Choriomeningitis virus: Negative
Mouse Adenovirus: Negative
Mouse Cytomegalovirus: Negative
Mouse Hepatitis Virus: Negative
Mouse Minute Virus: Negative
Mouse Norovirus: Negative
Mouse Parvovirus: Negative
Mouse Rotavirus: Negative
Mycoplasma Pulmonis: Negative
Pneumonia Virus of Mice: Negative
Polyoma Virus: Negative
Reovirus Screen: Negative
Sendai Virus: Negative
Theiler’s Murine Encephalomyelitis: Negative
Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

See the references for the original Armenian hamster IgG MR-1 antibody (https://bioxcell.com/catalogsearch/result/?q=BP0017-1).