Catalog #CP052
RecombiMAb anti-mouse CD38
Clone
NIMR5-CP052
Reactivities
Mouse
Isotype
Mouse IgG2a, κ
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Product Description
The NIMR5-CP052 monoclonal antibody is a chimeric version of the original NIMR5 antibody. The variable domain sequences are identical to the original NIMR5 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a. The NIMR5-CP052 monoclonal antibody reacts with mouse CD38 a 42 kDa type II transmembrane glycoprotein. CD38 is expressed on immature and mature resting and activated B cells as well as NK cells and a subset of T cells. CD38 expression is down-regulated on mouse germinal center B cells and plasma cells. CD38 is a multifunctional protein involved in NAD homeostasis and in the generation of the second messengers ADPR and cyclic ADPR which are involved in intracellular calcium signaling. Antibodies against CD38 have been shown to be highly efficacious in the treatment of multiple myeloma. The original NIMR5 antibody is reported as an agonistic antibody and has been shown to induce B cell proliferation, protect B cells from apoptosis, and slow disease progression in tumor-bearing mice.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | InVivoPlus mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | BCL1 plasma membrane glycoproteins |
| Reported Applications |
in vivo CD38 stimulation* in vitro CD38 stimulation* in vitro B cell activation* Immunofluorescence* ELISA* Flow cytometry* *Reported for the original rat IgG2a NIMR5 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |