Catalog #CP072
RecombiMAb anti-mouse CD172a (SIRPα) (LALA-PG)
Clone
P84-CP072
Reactivities
Mouse
Isotype
Mouse IgG2a
(switched from rat IgG1)
(switched from rat IgG1)
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Product Description
The P84-CP072 monoclonal antibody is a chimeric version of the original P84 antibody. The variable domain sequences are identical to the original P84 antibody but the constant region sequences have been switched from rat IgG1 to mouse IgG2a. The P84-CP072 antibody also contains a LALA-PG mutation in the Fc fragment rendering it unable to bind to endogenous Fcγ receptors. The P84-CP072 monoclonal antibody reacts with Signal-Regulatory Protein α (SIRPα), also known as CD172a. SIRPα is a type I transmembrane glycoprotein expressed on monocytes, macrophages, and dendritic cells. Neurons and other tissues of the central nervous system have also been shown to express SIRPα. Its ligand, CD47 is expressed by a wide variety of cells. SIRPα and CD47 regulate dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis. SIRPα diffuses laterally on the macrophage membrane and accumulates at a phagocytic synapse to bind CD47 which inhibits phagocytosis by macrophages. Anti-SIRPα antibodies that block the interaction of SIRPα with CD47 have been shown to suppress tumor formation in mice. The original P84 antibody has been shown to have neutralizing activity in vivo and in vitro.
Specifications
| Isotype | Mouse IgG2a, κ |
|---|---|
| Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Mouse brain membrane protein |
| Reported Applications |
in vivo SIRPα blocking* In vitro SIRPα blocking* Western blot* Immunoprecipitation* Flow cytometry* *Reported for the original rat IgG1 P84 antibody |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
≤0.5EU/mg (≤0.0005EU/μg) Determined by LAL gel clotting assay |
| Purity |
≥95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from HEK293 cell supernatant in an animal-free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
| Need a Custom Formulation? | See All Antibody Customization Options |